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Mouse Monoclonal CD45 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing PTPRC protein.

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Images

Flow Cytometry - PE Anti-CD45 antibody [2D1] (AB269297), expandable thumbnail
  • Flow Cytometry - PE Anti-CD45 antibody [2D1] (AB269297), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08575

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species

Human

Dilution info

10 µL for 106 Cells

Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

Alternative names

CD45, PTPRC, CD45, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

Recommended products

Mouse Monoclonal CD45 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing PTPRC protein.

Key facts

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08575
Clone number

2D1

Purification technique

Size-exclusion chromatography

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Flow Cytometry - PE Anti-CD45 antibody [2D1] (ab269297), expandable thumbnail

    Flow Cytometry - PE Anti-CD45 antibody [2D1] (ab269297)

    Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab269297 (10µl reagent / 100 µl of peripheral whole blood).

  • Flow Cytometry - PE Anti-CD45 antibody [2D1] (ab269297), expandable thumbnail

    Flow Cytometry - PE Anti-CD45 antibody [2D1] (ab269297)

    Flow cytometry surface staining pattern of human peripheral whole blood labeling CD45 with ab269297.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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