AB134202

PE Anti-CD45 antibody [MEM-28]

Name: Anti-CD45 PE antibody [MEM-28] (ab134202) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB134202
Price: 110 USD
Availability: InStock

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(9 Publications)

Mouse Monoclonal CD45 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images

Flow Cytometry - PE Anti-CD45 antibody [MEM-28] (AB134202)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD45 antibody [MEM-28] (AB134202)

Flow Cytometry analysis of human peripheral blood cells labeling CD45 with Anti-CD45 antibody [MEM-28], prediluted (Phycoerythrin) (ab134202).

Unconjugated

Anti-CD45 antibody [MEM-28]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD45 antibody [MEM-28]
660 APC

APC Anti-CD45 antibody [MEM-28]
Biotin

Biotin Anti-CD45 antibody [MEM-28]
675 PerCP

PerCP Anti-CD45 antibody [MEM-28]
778 PE/Cy7®

PE/Cy7® Anti-CD45 antibody [MEM-28]
785 APC/Cy7®

APC/Cy7® Anti-CD45 antibody [MEM-28]
618 PE/DyLight™ 594

PE/DyLight™ 594 Anti-CD45 antibody [MEM-28]
519 FITC

FITC Anti-CD45 antibody [MEM-28]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD45 antibody [MEM-28]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-28

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

Specificity

ab134202 reacts with all alternative forms of Human CD45.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

ab134202 is designed for Flow Cytometry analysis of Human blood cells using 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of proteome research 24:3578-3596 PubMed40526398

2025

Quantitative Proteomics Unveils the Synergistic Effects of Combination Drugs on Cytoskeleton Composition and Autophagy-Mediated Cell Death in Neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Chen Yu,Yi-Chun Kao,Hsin-Yi Chang,Chen-Hao Huang,Wen-Ming Hsu,Hsuan-Cheng Huang,Hsueh-Fen Juan

Nature communications 15:541 PubMed38225245

2024

Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Linnea Z Drexhage,Shengpan Zhang,Maeva Dupont,Franziska Ragaller,Ellen Sjule,Jose Cabezas-Caballero,Lachlan P Deimel,Helen Robertson,Rebecca A Russell,Omer Dushek,Erdinc Sezgin,Niloofar Karaji,Quentin J Sattentau

Cancer communications (London, England) 43:365-386 PubMed36739595

2023

μ-opioid receptor agonist facilitates circulating tumor cell formation in bladder cancer via the MOR/AKT/Slug pathway: a comprehensive study including randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqiang Wang,Song Zhang,Di Jin,Jiamei Luo,Yumiao Shi,Yiqi Zhang,Lingling Wu,Yanling Song,Diansan Su,Zhiying Pan,Haige Chen,Ming Cao,Chaoyong Yang,Weifeng Yu,Jie Tian

In vivo (Athens, Greece) 35:2127-2134 PubMed34182488

2021

Integrin α5β1 Mediated Cellular Reorganization in Human Mesenchymal Stem Cells During Neuronal Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Nihal Karakaş,Ülkan Kiliç

PloS one 15:e0228510 PubMed32407317

2020

Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity.

Applications

Unspecified application

Species

Unspecified reactive species

Nihal Karakaş,Sadık Bay,Nezaket Türkel,Nurşah Öztunç,Merve Öncül,Hülya Bilgen,Khalid Shah,Fikrettin Şahin,Gürkan Öztürk

Stem cells international 2019:2546367 PubMed31191669

2019

Exosomes from Human Gingiva-Derived Mesenchymal Stem Cells Combined with Biodegradable Chitin Conduits Promote Rat Sciatic Nerve Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Rao,Dianying Zhang,Tengjiaozi Fang,Changfeng Lu,Bo Wang,Xiao Ding,Shuai Wei,Yiran Zhang,Wei Pi,Hailin Xu,Yanhua Wang,Baoguo Jiang,Peixun Zhang

Journal of tissue engineering and regenerative med 13:433-445 PubMed30650247

2019

Effect of donor variation on osteogenesis and vasculogenesis in hydrogel cocultures.

Applications

Unspecified application

Species

Unspecified reactive species

Iris Pennings,Lukas A van Dijk,Juliet van Huuksloot,Joost O Fledderus,Koen Schepers,A Koen Braat,Edward C Hsiao,Emilie Barruet,Blanca M Morales,Marianne C Verhaar,Antoine J W P Rosenberg,Debby Gawlitta

Oncoimmunology 7:e1438111 PubMed29900038

2018

Dynamic change of PD-L1 expression on circulating tumor cells in advanced solid tumor patients undergoing PD-1 blockade therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Yue,Yubo Jiang,Ping Li,Yuehua Wang,Jian Xue,Nannan Li,Da Li,Ruina Wang,Yongjun Dang,Zhiyuan Hu,Yanlian Yang,Jianming Xu

Oncotarget 8:12917-12928 PubMed28039472

2017

Analysis of circulating tumor cells from lung cancer patients with multiple biomarkers using high-performance size-based microfluidic chip.

Applications

Unspecified application

Species

Unspecified reactive species

Wanlei Gao,Haojun Yuan,Fengxiang Jing,Shan Wu,Hongbo Zhou,Hongju Mao,Qinghui Jin,Jianlong Zhao,Hui Cong,Chunping Jia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD45 antibody [MEM-28]

Flow Cytometry - PE Anti-CD45 antibody [MEM-28] (AB134202)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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