AB25647

PE Anti-CD45RB antibody [C363.16A]

Name: PE Anti-CD45RB antibody [C363.16A] (ab25647) | Abcam
Brand: Abcam Limited
SKU: AB25647
Price: 160 USD
Availability: InStock

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(1 Publication)

Rat Monoclonal CD45 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Ptprc protein.

View Alternative Names

CD45, Ly-5, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, Lymphocyte antigen 5, T200, L-CA

1 Images

Flow Cytometry - PE Anti-CD45RB antibody [C363.16A] (AB25647)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD45RB antibody [C363.16A] (AB25647)

BALB/c splenocytes were double-stained with ab25647 (0.1µg for 10⁶ cells) and rat anti-mouse CD8a-FITC. Small lymphocytes were then gated and analyzed on a flow cytometer.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

C363.16A

Isotype

IgG2a

Light chain type

kappa

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Ptprc protein. The exact immunogen used to generate this antibody is proprietary information.

P06800

Epitope

ab25647 reacts with an exon B dependent epitope of CD45 glycoprotein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45RB also known as protein tyrosine phosphatase receptor type C (PTPRC) is a membrane-associated protein found prominently on all nucleated hematopoietic cell surfaces. This protein has a molecular mass that ranges between 180-220 kDa depending on the variable splicing. Importantly CD45RB is a component of the CD45 group playing an important role in regulating antigen receptor signaling in lymphocytes affecting both T-cell and B-cell function. Its expression occurs mainly in hematopoietic tissues such as the bone marrow lymph nodes and spleen.

Biological function summary

CD45RB functions as an integral transmembrane protein-tyrosine phosphatase and does not operate as part of a complex but rather acts directly on its substrates. It regulates the phosphorylation state of Src family kinases that are necessary for antigen receptor signaling. By modulating this phosphorylation CD45RB controls signal transduction influencing growth differentiation and immune response in lymphocytes. Its regulatory capacity extends to alterations in cell cycle progression and apoptosis maintaining homeostasis within the immune system.

Pathways

Studies link CD45RB to important pathways like the T-cell receptor signaling and B-cell receptor signaling pathways. These pathways are essential for the activation and differentiation of these immune cells. CD45RB acts in coordination with kinases such as Lck and Fyn (of the Src family) aiding in the dephosphorylation required for their activation. This interaction highlights CD45RB's fundamental role in fine-tuning responses to external antigens enabling appropriate immune responses.

CD45RB has associations particularly with autoimmune diseases and leukemia. Aberrant expression or function of CD45RB can lead to improper immune function contributing to conditions like multiple sclerosis and certain leukemias. Interactions with other membrane proteins including BRCA1 can also indirectly influence cancer dynamics through immune surveillance mechanisms. These associations make CD45RB a target of interest in developing therapeutic strategies for immune-dysregulation and hematological malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.

See full target information Ptprc

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of immunology 19:617-23 PubMed2567241

1989

A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

K Bottomly,M Luqman,L Greenbaum,S Carding,J West,T Pasqualini,D B Murphy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD45RB antibody [C363.16A]

Flow Cytometry - PE Anti-CD45RB antibody [C363.16A] (AB25647)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise