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AB275209

PE Anti-CD46 antibody [EPR23242-171]

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Rabbit Recombinant Monoclonal CD46 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD46, MCP, MIC10, Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen

1 Images
Flow Cytometry - PE Anti-CD46 antibody [EPR23242-171] (AB275209)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-CD46 antibody [EPR23242-171] (AB275209)

Flow cytometric overlay histogram showing wild-type HAP1 (Green line) and CD46 knockout HAP1 cells labelling CD46 with ab275209 (Red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab275209) (1x106 in 100μl at 0.04μg/ml; 1/12500 dilution) for 30 min at 4°C.

Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line CD46 knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23242-171

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/12500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD46 also known as membrane cofactor protein (MCP) is a type I membrane protein with a molecular weight around 50-70 kDa due to glycosylation variability. This protein serves as a regulatory component in the complement system which protects host cells from damage by complement components. CD46 is widely expressed on almost all nucleated human cells. It plays a role in inactivating C3b and C4b components of the complement cascade preventing autologous complement-mediated damage. The gene coding for CD46 is located on chromosome 1q32 a region known for encoding several complement regulatory proteins.
Biological function summary

Beyond its complement regulatory role CD46 acts in immune response modulation and reproduction. It forms a part of a protein complex that involves members such as decay-accelerating factor and complement receptor 1. CD46 also engages in intracellular signaling that affects T cell function and differentiation. In the reproductive context it participates in sperm-oocyte fusion and has a role in trophoblast fusion during placental formation.

Pathways

CD46 integrates into various signaling pathways including the complement and T cell receptor signaling pathways. In the complement pathway CD46 interacts with C3b and factor I promoting cleavage and inactivation of C3b leading to pathway regulation. Involvement in the T cell receptor pathway hints that CD46 signaling protects against overactive immune responses by promoting a switch to a regulatory T cell phenotype which can modulate immunity and prevent autoimmunity.

CD46 has connections with autoimmune conditions and infectious diseases. For instance alterations in CD46 expression or function can contribute to atypical hemolytic uremic syndrome where dysregulation of the complement pathway occurs. Additionally certain pathogens like Neisseria gonorrhoeae utilize CD46 as a receptor for infection. CD46’s interaction with proteins such as C3b connects it directly to complement-related conditions highlighting its importance in maintaining immune system balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.. (Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells.. (Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3.. (Microbial infection) Acts as a receptor for cultured Measles virus.. (Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6.. (Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes (PubMed : 11260136, PubMed : 11971006, PubMed : 7708671, PubMed : 9379894).
See full target information CD46
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