JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB130421

PE Anti-CD55 antibody [MEM-118]

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal DAF antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD55 protein.

View Alternative Names

CD55, CR, DAF, Complement decay-accelerating factor

1 Images
Flow Cytometry - PE Anti-CD55 antibody [MEM-118] (AB130421)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD55 antibody [MEM-118] (AB130421)

Flow cytometry analysis (surface staining) of human peripheral blood cells labeling CD55 with ab130421.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-118

Isotype

IgM

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD55 protein. The exact immunogen used to generate this antibody is proprietary information.

P08174

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>10 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 8 Preservative: 0.1% Sodium azide Constituents: 97% PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD55 also known as Decay-Accelerating Factor (DAF) is a glycoprotein that regulates the complement cascade. This protein typically weighs around 70 kDa. It is expressed in many cells including epithelial and endothelial cells and is often present on the surface of blood cells. CD55 plays an important role in protecting cells against lysis by inhibiting the formation and stability of C3 and C5 convertases which ultimately prevent cell destruction by the complement system.
Biological function summary

CD55 helps maintain immune homeostasis by preventing the complement-mediated damage to host tissues. It functions as a member of the regulators of complement activation (RCA) family. While not necessarily part of a larger protein complex its function is related to other regulatory proteins such as CD46 and CD59 which cooperate to regulate complement activity. The DAF protein's immunology function is instrumental in ensuring that self-cells are not inadvertently attacked by the body's immune system.

Pathways

CD55 significantly impacts the complement pathway which is a central component of the innate immune system. It directly interacts with complement components C3 and C5 accelerating their decay and disassembly. Through this pathway CD55 has a working relationship with other complement regulatory proteins like Factor H which also aids in controlling complement activation on the cell surface.

A dysfunctional CD55 can relate to conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). In PNH the absence or insufficiency of CD55 (and CD59) on red blood cells leads to complement-mediated lysis causing hemolytic anemia. In aHUS impaired regulation of the complement system including abnormalities in CD55 function exacerbates symptoms. CD55's role is therefore important in preventing these complement-related disorders and preserving cellular integrity in the human body.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed : 7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed : 28657829).. (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5.. (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable).. (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.
See full target information CD55
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB231061

Anti-CD55 antibody

Western blot - Anti-CD55 antibody (AB231061)

  • 0
  • 0
Primary Antibodies

AB133684

Anti-CD55 antibody [EPR6689]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] (AB133684)

  • 5
  • 1
Primary Antibodies

AB243560

Anti-CD55 antibody [EPR22362-255] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD55 antibody [EPR22362-255] - BSA and Azide free (AB243560)

  • 0
  • 0
Primary Antibodies

AB230797

Anti-CD55 antibody [EPR6689] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

  • 0
  • 0
Primary Antibodies

AB243231

Anti-CD55 antibody [EPR22362-255]

RabMAb|Recombinant

Flow Cytometry - Anti-CD55 antibody [EPR22362-255] (AB243231)

  • 0
  • 0

Complementary Products

Assay Kits

AB256405

Human DAF ELISA Kit (CD55)

Recombinant|SimpleStep

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

  • 0
  • 0
Primary Antibodies

AB33257

PE Anti-HLA Class I antibody [W6/32]

  • 0
  • 0
Primary Antibodies

AB307169

Alexa Fluor® 647 Anti-CD59 antibody [EPR22394-242]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 647 Anti-CD59 antibody [EPR22394-242] (AB307169)

  • 0
  • 0
Primary Antibodies

AB283260

Anti-HLA G antibody [EPR23298-97]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [EPR23298-97] (AB283260)

  • 0
  • 0
Primary Antibodies

AB306574

Alexa Fluor® 647 Anti-CD33 antibody[EPR23052-5]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 647 Anti-CD33 antibody[EPR23052-5] (AB306574)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com