AB36421

PE Anti-CD59 antibody [MEM-43]

Name: Anti-CD59 PE antibody [MEM-43] (ab36421) Mouse monoclonal IgG2a | Abcam
Brand: Abcam Limited
SKU: AB36421

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(1 Publication)

Mouse Monoclonal CD59 antibody - conjugated to PE. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD59 protein.

View Alternative Names

CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL

3 Images

Immunocytochemistry/ Immunofluorescence - PE Anti-CD59 antibody [MEM-43] (AB36421)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - PE Anti-CD59 antibody [MEM-43] (AB36421)

ab36421 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab36421 at 1 in 100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Flow Cytometry - PE Anti-CD59 antibody [MEM-43] (AB36421)

Flow Cyt

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Flow Cytometry - PE Anti-CD59 antibody [MEM-43] (AB36421)

Flow Cytometry analysis of HL-60 (positive) and SP2 (negative) cells labeling CD59 with Anti-CD59 antibody [MEM-43] (Phycoerythrin) (ab36421).

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD59 antibody [MEM-43] (AB36421)

ab36421 (concentration in sample 1 ug/ml, red-filled histogram) binds specifically to CD59 expressed on surface of HL60 cells (upper panel), but not to surface of SP2 cells (lower panel).

Level of non-specific binding was assessed using Mouse IgG2a isotype control PE antibody (PPV-04) under same conditions (concentration in sample 1 µg/ml, black-dashed histogram).

Unconjugated

Anti-CD59 antibody [MEM-43]
Biotin

Biotin Anti-CD59 antibody [MEM-43]
660 APC

APC Anti-CD59 antibody [MEM-43]
519 FITC

FITC Anti-CD59 antibody [MEM-43]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD59 antibody [MEM-43]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-43

Isotype

IgG2a

Light chain type

kappa

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Cell preparation containing CD59 protein. The exact immunogen used to generate this antibody is proprietary information.

P13987

Epitope

ab36421 reacts with the well defined epitope (W40, R-53) on the CD59 molecule.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "(or 100μl of whole blood).<a href='/en-us/products/primary-antibodies/pe-mouse-igg2a-b12-8-isotype-control-ab91363'>ab91363</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification notes

The conjugate is purified by size-exclusion chromatography.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.

Biological function summary

One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.

Pathways

CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.

Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.

See full target information CD59

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 31:1599-608 PubMed21860420

2011

Cellular features of senescence during the evolution of human and murine ductal pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

M E Caldwell,G M DeNicola,C P Martins,M A Jacobetz,A Maitra,R H Hruban,D A Tuveson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD59 antibody [MEM-43]

Immunocytochemistry/ Immunofluorescence - PE Anti-CD59 antibody [MEM-43] (AB36421)

Flow Cytometry - PE Anti-CD59 antibody [MEM-43] (AB36421)

Flow Cytometry - PE Anti-CD59 antibody [MEM-43] (AB36421)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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