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Rabbit Recombinant Monoclonal CD62E antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

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Images

Flow Cytometry - PE Anti-CD62E antibody [EPR25616-62] (AB322672), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - PE Anti-CD62E antibody [EPR25616-62] (AB322672), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/250

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Associated Products

Select an associated product type

1 product for Isotype control

Target data

Function

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Alternative names

Rabbit Recombinant Monoclonal CD62E antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR25616-62

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD62E also known as E-selectin or ELAM-1 is a cell adhesion molecule with a mass of approximately 115 kDa. It is present mainly on endothelial cells activated by cytokines. As a transmembrane glycoprotein E-selectin plays a mechanical role in mediating the tethering and rolling of leukocytes on the vascular endothelium during the inflammatory response. This function is important in directing leukocytes to sites of tissue damage or infection.

Biological function summary

E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.

Pathways

CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.

Associated diseases and disorders

E-selectin expression is highly relevant in inflammatory diseases such as rheumatoid arthritis and atherosclerosis. These disorders involve chronic inflammation where the persistent activation of endothelial cells and overexpression of E-selectin contribute to pathology. The link with ICAM-1 in these settings suggests a mutual regulation between cell adhesion molecules enhancing leukocyte recruitment to inflamed tissues. This makes E-selectin not only a marker of endothelial activation but also a potential therapeutic target for modulating leukocyte adhesion in inflammatory diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - PE Anti-CD62E antibody [EPR25616-62] (ab322672), expandable thumbnail

    Flow Cytometry - PE Anti-CD62E antibody [EPR25616-62] (ab322672)

    Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell) treated with 20ng/ml IL-lbeta for 4 hours (Right) / Untreated control (Left) cells labelling CD62E with ab322672 at 1/500 dilution (0.1ug) / Right and Left compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - PE Anti-CD62E antibody [EPR25616-62] (ab322672), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - PE Anti-CD62E antibody [EPR25616-62] (ab322672)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling CD62E with ab322672 at 1/250 (2.0 ug/ml) dilution (Green).

    Confocal image showing increased cytoplasmic and membranous staining in HUVEC cells treated with interleukin-lbeta (20 ng/ml) for 4 h (shown in magenta) (PMID: 16715142). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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