AB119104

PE Anti-P-Selectin (CD62P) antibody [AK4]

Name: PE Anti-P-Selectin (CD62P) antibody [AK4] (ab119104) | Abcam
Brand: Abcam Limited
SKU: AB119104
Price: 110 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Anti-P-Selectin (CD62P) antibody [AK4] conjugated to PE - suitable for flow cytometry, and reacts with human samples. Clone [AK4] is a mouse monoclonal antibody cited in 48 publications.

Design your panel with our interactive flow cytometry panel builder. Click here.

1 Images

Flow Cytometry - PE Anti-P-Selectin (CD62P) antibody [AK4] (AB119104)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-P-Selectin (CD62P) antibody [AK4] (AB119104)

Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD62P (AK4) PE. 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.

660 APC

APC Anti-P-Selectin (CD62P) antibody [AK4]
Biotin

Biotin Anti-P-Selectin (CD62P) antibody [AK4]
519 FITC

FITC Anti-P-Selectin (CD62P) antibody [AK4]
695 PE/Cy5.5®

PE/Cy5.5® Anti-P-Selectin (CD62P) antibody [AK4]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AK4

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing SELP protein. The exact immunogen used to generate this antibody is proprietary information.

P16109

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension.<a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension.<a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

ab119104 is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62P also known as P-selectin is a cell adhesion molecule expressed on the surface of activated endothelial cells and platelets. It is a type of protein with a known molecular weight of approximately 140 kDa. P-selectin plays a mechanical role in mediating leukocyte rolling on the endothelium which is an initial step in the inflammatory response. This protein is rapidly translocated to the plasma membrane upon platelet activation and is stored in alpha granules of platelets and Weibel-Palade bodies of endothelial cells.

Biological function summary

P-selectin is critical in the recruitment of leukocytes to sites of tissue injury and infection. It facilitates the adhesion of leukocytes to the vascular endothelium by binding to its ligand PSGL-1 on the leukocyte surface. This interaction supports the transition of leukocytes from the circulation to the site of inflammation. P-selectin is also involved in thrombus formation as it contributes to the interactions between platelets leukocytes and endothelium in the vascular wall. It acts as part of a dynamic complex in the regulation of the innate immune response and hemostasis.

Pathways

CD62P plays an important role in both the inflammatory and coagulation pathways. It is an important mediator in the leukocyte adhesion cascade influencing leukocyte tethering and rolling on activated endothelium. The interaction of P-selectin with PSGL-1 initiates the signaling pathways that lead to firm adhesion and extravasation of leukocytes. In the coagulation pathway P-selectin enhances interactions between platelets and leukocytes which support thrombus formation. Its regulation and activity are tightly linked with other proteins such as E-selectin and L-selectin in coordinating vascular and systemic inflammatory responses.

Altered expression of CD62P is associated with various inflammatory conditions and cardiovascular diseases. In thrombosis increased levels of P-selectin enhance platelet aggregation and stability of the thrombus contributing to pathological clot formation. Similarly in atherosclerosis P-selectin facilitates the recruitment of monocytes to the vessel wall promoting plaque development. The protein's function relates to interactions with key inflammatory mediators and its dysregulation is often observed in conditions like chronic inflammatory diseases and acute myocardial infarction.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B : ITGB3) and integrin alpha-V/beta-3 (ITGAV : ITGB3) (PubMed : 37184585).

See full target information SELP

Alternative Names

CD62P, GMRP, GRMP, SELP, P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane protein, GMP-140, LECAM3, PADGEM

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 175:1147-50 PubMed1372646

1992

Characterization of GMP-140 (P-selectin) as a circulating plasma protein.

Applications

Unspecified application

Species

Unspecified reactive species

L C Dunlop,M P Skinner,L J Bendall,E J Favaloro,P A Castaldi,J J Gorman,J R Gamble,M A Vadas,M C Berndt

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-P-Selectin (CD62P) antibody [AK4]

Flow Cytometry - PE Anti-P-Selectin (CD62P) antibody [AK4] (AB119104)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise