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AB322728

PE Anti-CD63 antibody [KILL150A]

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Mouse Recombinant Monoclonal CD63 antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt, IHC-P and reacts with Human samples.

View Alternative Names

CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30

4 Images
Immunocytochemistry/ Immunofluorescence - PE Anti-CD63 antibody [KILL150A] (AB322728)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Anti-CD63 antibody [KILL150A] (AB322728)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling CD63 with ab322728 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in HUVEC cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab185031 Recombinant Alexa Fluor® 488 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab185031) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Flow Cytometry - PE Anti-CD63 antibody [KILL150A] (AB322728)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD63 antibody [KILL150A] (AB322728)

Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell) cells labelling CD63 with ab322728 at 1/50 dilution (1ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.

Flow Cytometry - PE Anti-CD63 antibody [KILL150A] (AB322728)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD63 antibody [KILL150A] (AB322728)

Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / SK-MEL-28 (Human malignant melanoma, Right) cells labelling CD63 with ab322728 at 1/5000 dilution (0.01ug) (Red) compared with a Mouse IgG isotype control - Phycoerythrin (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.
Low expression : Jurkat.

Immunocytochemistry/ Immunofluorescence - PE Anti-CD63 antibody [KILL150A] (AB322728)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - PE Anti-CD63 antibody [KILL150A] (AB322728)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-MEL-28 (human malignant melanoma cell) cells labelling CD63 with ab322728 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in SK-MEL-28 cell line(shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue).
Low expression : Jurkat
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab185031 Recombinant Alexa Fluor® 488 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab185031) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KILL150A

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>&lt;p&gt;We recommend ab134045 for low expression samples.&lt;/p&gt;<br><br>&nbsp;</p>

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Complementary Products

Assay Kits

AB275099

Human CD63 ELISA Kit

Recombinant|SimpleStep

ELISA - Human CD63 ELISA Kit (AB275099)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.
Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
See full target information CD63
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com