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AB157335

PE Anti-CD73 antibody [AD2]

1

(1 Review)

|

(11 Publications)

Mouse Monoclonal CD73 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 11 publications.

View Alternative Names

CD73, NT5, NTE, NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase

1 Images
Flow Cytometry - PE Anti-CD73 antibody [AD2] (AB157335)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD73 antibody [AD2] (AB157335)

Surface staining (flow cytometry) of human peripheral blood with anti-human CD73 PE (ab157335).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD2

Isotype

IgG1

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

ab157335 recognizes CD73 expressed predominantly on T and B cell subsets, follicular dendritic cells and endothelial cells

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 10μl per 100μl whole blood. The content of a vial (1 ml) is sufficient for 50 tests.<br><a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
ab157335 is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: 99.7% PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD73 also known as ecto-5'-nucleotidase is an enzyme that functions at the cellular level. The CD73 protein hydrolyzes extracellular nucleotides into nucleosides by removing phosphate groups. It is a 70 kDa glycosylphosphatidylinositol-anchored molecule found on the surface of various cell types including endothelial cells lymphocytes and epithelial cells. Its presence is noted in a wide range of tissues across the human body highlighting its versatile role in cellular communication and metabolism.
Biological function summary

CD73 participates in the regulation of purinergic signaling through adenosine production. It acts in cooperation with other cell surface enzymes such as CD39 forming a functional complex that handles ATP breakdown to adenosine. This enzymatic activity modulates immune responses tissue protection and inflammation control. In addition to immune-related functions CD73 supports the maintenance of vascular integrity and proper cellular adhesion which is important in different physiological and pathological contexts.

Pathways

CD73 plays an important part in the adenosinergic pathway governing the conversion of AMP into adenosine. This activity impacts the signaling and function of the adenosine receptors on immune and non-immune cells. Another important pathway is the angiogenesis process where CD73 contributes by affecting endothelial cell migration and blood vessel development. Relationships with key proteins such as adenosine A2 receptors help facilitate these critical pathway activities.

CD73's involvement with immunosuppression and tumor progression becomes significant. Elevated levels of CD73 are frequently observed in some cancers where it aids in creating a favorable tumor microenvironment through increased adenosine production. This situation assists tumor cells in evading immune detection. Additionally CD73 is linked to autoimmune diseases like multiple sclerosis where it contributes to modulated lymphocyte activity. Connections with proteins such as TGF-beta further illustrate its role in disease-related pathways creating potential targets for therapeutic interventions through the use of anti-CD73 antibodies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed : 34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084).
See full target information NT5E
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 35:101497 PubMed37534324

2023

Umbilical mesenchymal stem cell-derived exosomes promote spinal cord functional recovery through the miR-146b/TLR4 -mediated NF-κB p65 signaling pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiujuan Wang,Ying Yang,Wei Li,MingYuan Hao,YongSheng Xu

Animals : an open access journal from MDPI 13: PubMed36611625

2022

Isolation and Identification of Bone Marrow Mesenchymal Stem Cells from Forest Musk Deer.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqiang Luo,Yangyang Geng,Mengxi Gao,Mengting Cao,Junjian Wang,Jing Yang,Chenxuan Sun,Xingrong Yan

Turkish journal of biology = Turk biyoloji dergisi 46:400-413 PubMed37529007

2022

The investigation of diverse physiological and therapeutic impact of cellular-based products derived from human cumulus cells.

Applications

Unspecified application

Species

Unspecified reactive species

Derya Burukçu,Sema Yilmaz,Oya Alagöz,Cumhur Kaan Yaltirik,Erkut Attar,Fikrettin Şahin,Esra Aydemir

Cell death discovery 8:269 PubMed35606376

2022

Transfer of microRNA-22-3p by M2 macrophage-derived extracellular vesicles facilitates the development of ankylosing spondylitis through the PER2-mediated Wnt/β-catenin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Liu,Tuo Liang,Zide Zhang,Jiarui Chen,Jang Xue,Xinli Zhan,Liang Ren

Bioengineered 12:12891-12904 PubMed34784837

2021

MiR-455-5p upregulation in umbilical cord mesenchymal stem cells attenuates endometrial injury and promotes repair of damaged endometrium via Janus kinase/signal transducer and activator of transcription 3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyan Sun,Zhihe Jiang,Yanling Chen,Di Shang,Pan Miao,Jian Gao

Journal of immunology research 2021:2389029 PubMed34692851

2021

Lysophosphatidylcholine Offsets the Protective Effects of Bone Marrow Mesenchymal Stem Cells on Inflammatory Response and Oxidative Stress Injury of Retinal Endothelial Cells via TLR4/NF-B Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Haijun Zhao,Yanhui He

In vivo (Athens, Greece) 35:2127-2134 PubMed34182488

2021

Integrin α5β1 Mediated Cellular Reorganization in Human Mesenchymal Stem Cells During Neuronal Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Nihal Karakaş,Ülkan Kiliç

PloS one 16:e0252575 PubMed34133442

2021

Novel low shear 3D bioreactor for high purity mesenchymal stem cell production.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew B Burns,Corinna Doris,Kevin Vehar,Vinit Saxena,Cameron Bardliving,Parviz A Shamlou,M Ian Phillips

Frontiers in pharmacology 12:613449 PubMed33867979

2021

Abacavir Increases Purinergic P2X7 Receptor Activation by ATP: Does a Pro-inflammatory Synergism Underlie Its Cardiovascular Toxicity?

Applications

Unspecified application

Species

Unspecified reactive species

Víctor Collado-Díaz,Maria Ángeles Martinez-Cuesta,Maria Amparo Blanch-Ruiz,Ainhoa Sánchez-López,Patricia García-Martínez,José E Peris,Iris Usach,Maria Dolores Ivorra,Alessandra Lacetera,Sonsoles Martín-Santamaría,Juan V Esplugues,Angeles Alvarez

PloS one 15:e0228510 PubMed32407317

2020

Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity.

Applications

Unspecified application

Species

Unspecified reactive species

Nihal Karakaş,Sadık Bay,Nezaket Türkel,Nurşah Öztunç,Merve Öncül,Hülya Bilgen,Khalid Shah,Fikrettin Şahin,Gürkan Öztürk
View all publications
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