AB22548

PE Anti-CD8 alpha antibody [MIL-12]

Name: PE Anti-CD8 alpha antibody [MIL-12] (ab22548) | Abcam
Brand: Abcam Limited
SKU: AB22548
Price: 525 USD
Availability: InStock

(1 Review)

(1 Publication)

Mouse Monoclonal CD8 alpha antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Pig samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD8 alpha protein.

3 Images

Flow Cytometry - PE Anti-CD8 alpha antibody [MIL-12] (AB22548)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD8 alpha antibody [MIL-12] (AB22548)

Flow cytometry staining of red cell lysed porcine blood with ab22548.

Figure A. FITC conjugated Mouse anti Porcine CD45a antibody and RPE conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated Mouse anti Porcine CD45a antibody and RPE conjugated Mouse anti Porcine CD8a antibody, clone MIL12 (ab22548).

All experiments performed on red cell lysed porcine blood (10ul neat to label 1x10⁶ cells in 100ul) gated on lymphocytes.

Data acquired on the ZE5 Cell analyser.

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD8 alpha antibody [MIL-12] (AB22548)

Flow cytometry staining of red cell lysed porcine blood with ab22548.

Figure A. FITC conjugated Mouse anti Porcine CD5 antibody and RPE conjugated mouse IgG1 isotype control. Figure B. FITC conjugated Mouse anti Porcine CD5 antibody and RPE conjugated Mouse anti Porcine CD8a antibody, clone MIL12 (ab22548).

All experiments performed on red cell lysed porcine blood (10ul neat to label 1x10⁶ cells in 100ul) gated on mononuclear cells.

Data acquired on the ZE5 Cell analyser.

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD8 alpha antibody [MIL-12] (AB22548)

Flow cytometry staining of red cell lysed porcine blood with ab22548.

Figure A. RPE conjugated Mouse anti Porcine CD8a antibody, clone MIL12 (ab22548) and FITC conjugated Mouse IgG2a isotype control. Figure B. RPE conjugated Mouse anti Porcine CD8a antibody, clone MIL12 (ab22548) and FITC conjugated Mouse anti Porcine CD5 antibody.

All experiments performed on red cell lysed porcine blood (10ul neat to label 1x10⁶ cells in 100ul) gated on mononuclear cells.

Data acquired on the ZE5 Cell analyser.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MIL-12

Isotype

IgG2a

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Pig

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognises a subset of porcine T lymphocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Pig": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-mouse-igg2a-b12-8-isotype-control-ab91363'>ab91363</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Purified IgG conjugated to R. Phycoerythrin (RPE).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information CD8A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 12:7425-32 PubMed26459920

2015

Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Zhou,Xiaoyong Li,Meina Feng,Qi Zhang,Zhendong Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD8 alpha antibody [MIL-12]

Flow Cytometry - PE Anti-CD8 alpha antibody [MIL-12] (AB22548)

Flow Cytometry - PE Anti-CD8 alpha antibody [MIL-12] (AB22548)

Flow Cytometry - PE Anti-CD8 alpha antibody [MIL-12] (AB22548)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise