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AB306536

PE Anti-CD8 antibody [EPR21769]

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Rabbit Recombinant Monoclonal CD8 alpha antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD8a, Lyt-2, Lyt2, Cd8a, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2

1 Images
Flow Cytometry - PE Anti-CD8 antibody [EPR21769] (AB306536)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-CD8 antibody [EPR21769] (AB306536)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab306536 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 μg/ml anti CD16/CD32 and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab306536) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (1x 106 in 100μl at 1 μg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD4.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live lymphocytes.

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD8 alpha antibody [EPR21769]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD8 alpha antibody [EPR21769]

  • 519 FITC

    FITC Anti-CD8 alpha antibody [EPR21769]

  • 660 APC

    APC Anti-CD8 alpha antibody [EPR21769]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD8 alpha antibody [EPR21769]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

  • Unconjugated

    Anti-CD8 alpha antibody [EPR21769]

  • Carrier free

    Anti-CD8 alpha antibody [EPR21769] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21769

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD8 also known as CD8a or Leu-2 is a glycoprotein with a molecular weight of approximately 32 kDa. It is prominently expressed on the surface of cytotoxic T lymphocytes. These CD8 molecules facilitate the interaction between T cells and antigen-presenting cells by binding to major histocompatibility complex (MHC) class I molecules. This interaction is essential for the immune system to recognize and destroy infected or cancerous cells. CD8 is often identified using various markers like CD8 FITC CD8 APC and CD8 PerCP which are used in flow cytometry for cell sorting and analysis.
Biological function summary

CD8 plays a significant role in the adaptive immune response. It is not part of a multimeric complex but functions as a co-receptor that enhances the recognition of antigens by the T cell receptor (TCR). The presence of CD8 molecules aids the activation of T cells leading to their proliferation and the effective targeting and elimination of pathogens or abnormal cells. The ability of CD8 to bind to MHC class I molecules allows cytotoxic T cells to detect and respond to nearly all nucleated cells in the body.

Pathways

CD8 is integral in both the T cell receptor signaling pathway and the induction of apoptosis. In the T cell receptor signaling pathway CD8 works closely with other proteins like Lck and ZAP-70 to transmit signals upon TCR-MHC class I engagement. This promotes T cell activation cytokine production and cell division. Furthermore CD8's interaction with Fas ligand pathways contributes to the initiation of programmed cell death which is important for maintaining immune homeostasis and preventing overreaction of the immune system.

CD8 T cells are significant in the context of viral infections and autoimmune diseases. For example during HIV infection the depletion of CD8 T cells impairs the immune response allowing the virus to evade the immune system. Additionally in autoimmune disorders like Type 1 diabetes CD8 T cells mistakenly target and destroy insulin-producing beta cells in the pancreas. These pathological processes also involve proteins such as MHC class I and other immune modulators like interleukins that modulate CD8 T cell activity and differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule : peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
See full target information Cd8a
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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