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Mouse Monoclonal CD8 beta antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Rat samples. Immunogen corresponding to Cell preparation containing Cd8b protein.

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Images

Flow Cytometry - PE Anti-CD8 beta antibody [341] (AB95526), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing Cd8b protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05541

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Rat
Tested

Tested
Tested

Species
Rat
Dilution info
0.25 µg for 105-108 Cells
Notes

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Associated Products

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1 product for Alternative Version

Target data

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells.

Alternative names

CD8b, Cd8b1, Cd8b, T-cell surface glycoprotein CD8 beta chain, CD8 antigen 37 kDa chain, OX-8 membrane antigen

Recommended products

Mouse Monoclonal CD8 beta antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Rat samples. Immunogen corresponding to Cell preparation containing Cd8b protein.

Key facts

Isotype
IgG1
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing Cd8b protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05541
Clone number
341
Purification technique
Affinity purification Protein G
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Excitation: 488 nm Emission: 575 nm.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The target CD8 beta also known as CD8b or CD8B is a protein component of the CD8 coreceptor that is primarily expressed on the surface of cytotoxic T lymphocytes and a subset of natural killer cells. The CD8 beta molecule pairs with CD8 alpha to form a heterodimer that plays an essential role in the immune response. The molecular weight of CD8 beta is approximately 34 kDa. It is located primarily in the plasma membrane where it interacts with major histocompatibility complex (MHC) class I molecules.

Biological function summary

The CD8 beta protein works as part of the CD8 coreceptor complex which enhances the interaction between T cell receptors (TCRs) and antigens presented by MHC class I on infected or malignant cells. This interaction strengthens the activation signal received by T cells. The CD8 complex including CD8 beta contributes to the recognition and elimination of cells presenting foreign antigens which is essential for the adaptive immune response.

Pathways

The CD8 beta protein participates in pathways related to antigen recognition and T cell receptor signaling. The protein together with CD8 alpha facilitates signal transduction following TCR engagement. This action is vital for the activation of cytotoxic T cells enabling immune responses. CD8 beta associates with the Lck kinase another vital player in the TCR signaling pathway through its cytoplasmic tail. In this pathway the presence of CD8 beta helps maintain and modulate effective immune responses.

Associated diseases and disorders

CD8 beta plays a significant role in conditions like viral infections and autoimmune diseases. In viral infections the presence of the CD8 complex containing CD8 beta aids in the destruction of virus-infected cells. In autoimmune diseases abnormal CD8 T cell responses can result in self-tissue damage reflecting dysregulation in its normal function. CD8 beta therefore proves critical in these contexts contributing to disease dynamics by interacting with proteins like MHC class I and Lck kinase during improper immune responses.

Product promise

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1 product image

  • Flow Cytometry - PE Anti-CD8 beta antibody [341] (ab95526), expandable thumbnail

    Flow Cytometry - PE Anti-CD8 beta antibody [341] (ab95526)

    Staining of rat splenocytes with 0.125 μg of Mouse IgG1 kappa Isotype Control PE (open histogram) or 0.125 μg of ab95526 (filled histogram). Cells in the lymphocyte gate were used for analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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