AB93507

PE Anti-CD80 antibody [16-10A1]

Name: Anti-CD80 PE antibody [16-10A1] (ab93507) Hamster monoclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB93507
Price: 170 USD
Availability: InStock

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(7 Publications)

Armenian hamster Monoclonal CD80 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing Cd80 protein.

View Alternative Names

CD80, B7, Cd80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen

1 Images

Flow Cytometry - PE Anti-CD80 antibody [16-10A1] (AB93507)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD80 antibody [16-10A1] (AB93507)

Flow Cytometry analysis of C57Bl/6 splenocytes unstimulated (left panel) or stimulated for 3 days with LPS (right panel) labeling CD80 with ab93507 at 0.125μg (solid line). Isotype control - Armenian Hamster IgG (Phycoerythrin) 0.125μg.

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

16-10A1

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd80 protein. The exact immunogen used to generate this antibody is proprietary information.

Q00609

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>ab101026 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze|Store undiluted

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.

Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed : 22450810).

See full target information Cd80

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 18:1469-1489 PubMed36998601

2023

Mitochondrial Calcium Nanoregulators Reverse the Macrophage Proinflammatory Phenotype Through Restoring Mitochondrial Calcium Homeostasis for the Treatment of Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Lei,Guodong Tan,Yiming Wang,Li Chen,Yuan Cao,Bingxin Si,Zhen Zhen,Bei Li,Yan Jin,Wei Wang,Fang Jin

Nature communications 13:5700 PubMed36171212

2022

In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyan Zhou,Mengchao Yu,Luzhen Ma,Jinyu Fu,Jingwei Guo,Jieqiong Lei,Zheng Fu,Yong Fu,Qipeng Zhang,Chen-Yu Zhang,Xi Chen

Frontiers in immunology 13:828263 PubMed35251013

2022

Development of a Dendritic Cell/Tumor Cell Fusion Cell Membrane Nano-Vaccine for the Treatment of Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Wei Zhao,Jinke Huang,Fangxuan Li,Jindong Sheng,Hualin Song,Ying Chen

International journal of biological macromolecules 161:797-809 PubMed32553971

2020

Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shengnan Liu,Ye Yang,Yuan Qu,Xiaoxi Guo,Xiaoyan Yang,Xiuming Cui,Chengxiao Wang

The Journal of biological chemistry 281:38617-24 PubMed17046818

2006

NF-kappaB2 is required for the control of autoimmunity by regulating the development of medullary thymic epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Baochun Zhang,Zhe Wang,Jane Ding,Pärt Peterson,William T Gunning,Han-Fei Ding

Journal of the American Society of Nephrology : JA 16:2012-22 PubMed15944341

2005

Glomerular expression of CD80 and CD86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Dragana Odobasic,A Richard Kitching,Timothy J Semple,Jennifer R Timoshanko,Peter G Tipping,Stephen R Holdsworth

Proceedings of the National Academy of Sciences of the United States of America 93:13967-72 PubMed8943044

1996

Costimulatory function and expression of CD40 ligand, CD80, and CD86 in vascularized murine cardiac allograft rejection.

Applications

Unspecified application

Species

Unspecified reactive species

W W Hancock,M H Sayegh,X G Zheng,R Peach,P S Linsley,L A Turka

View all publications

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Product promise

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PE Anti-CD80 antibody [16-10A1]

Flow Cytometry - PE Anti-CD80 antibody [16-10A1] (AB93507)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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