AB33694

PE Anti-CD90 / Thy1 antibody [MRC OX-7]

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(17 Publications)

Mouse Monoclonal CD90 / Thy1 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Rat, Mouse samples. Cited in 17 publications.

View Alternative Names

CD90, Thy-1, Thy1, Thy-1 membrane glycoprotein, Thy-1 antigen

1 Images

Flow Cytometry - PE Anti-CD90 / Thy1 antibody [MRC OX-7] (AB33694)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD90 / Thy1 antibody [MRC OX-7] (AB33694)

Flow cytometry staining of red cell lysed rat blood with ab33694.

Figure A. FITC conjugated Mouse anti Rat CD3 and PE conjugated Mouse IgG1 isotype control. Figure B. FITC conjugated Mouse anti Rat CD3 and PE conjugated Mouse anti Rat CD90 (ab33694).

All experiments performed on red cell lysed rat blood gated on lymphoid cells in the presence of 10% rat serum (10ul neat to label 1x10⁶ cells in 100ul).

Data acquired on the ZE5 Cell Analyzer.

519 FITC

FITC Anti-CD90 / Thy1 antibody [MRC OX-7]
HRP

HRP Anti-CD90 / Thy1 antibody [MRC OX-7]
675 PerCP

PerCP Anti-CD90 / Thy1 antibody [MRC OX-7]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MRC OX-7

Isotype

IgG1

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Mouse, Rat

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Since CD90 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of MRC OX-7 for rat Thy1 is 3 x 109m-1 and for mouse Thy1.1 is 3 x 108m-1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "or 100ul whole blood. <a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "or 100ul whole blood. <a href='/en-us/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Product details

ab33694 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

See full target information Thy1

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering 15:20417314241306681 PubMed39712077

2024

Harnessing bone marrow mesenchymal stem cell-derived extracellular vesicles and biomimetic peptide WKYMVm in self-healing hydrogel for enhanced bone repair in femoral defects.

Applications

Unspecified application

Species

Unspecified reactive species

Keyu Luo,Yufei Jin,Baiyi Liu,Yingbo Wang,Yaoyao Liu,Si Qiu,Jianhua Zhao,Xiang Yin

Molecular medicine reports 31: PubMed39540371

2024

Dental pulp stem cell‑derived extracellular vesicles loaded with hydrogels promote osteogenesis in rats with alveolar bone defects.

Applications

Unspecified application

Species

Unspecified reactive species

Xin He,Xiao-Yang Chu,Xu Chen,Yu-Lan Xiang,Ze-Lu Li,Chun-Yan Gao,Ying-Yi Luan,Kai Yang,Dong-Liang Zhang

Journal of the American Heart Association 12:e029131 PubMed37345832

2023

Electron Leak From the Mitochondrial Electron Transport Chain Complex I at Site I Is Crucial for Oxygen Sensing in Rabbit and Human Ductus Arteriosus.

Applications

Unspecified application

Species

Unspecified reactive species

Austin D Read,Rachel E T Bentley,Ashley Y Martin,Jeffrey D Mewburn,Elahe Alizadeh,Danchen Wu,Patricia D A Lima,Kimberly J Dunham-Snary,Bernard Thébaud,Willard Sharp,Stephen L Archer

BioMed research international 2022:8537959 PubMed36119932

2022

VEGFA-Enriched Exosomes from Tendon-Derived Stem Cells Facilitate Tenocyte Differentiation, Migration, and Transition to a Fibroblastic Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaowen Xue,Zihang Chen,Tingting Wu,Riwang Li,Chao Chen,Junting Liu,Huige Hou,Xiaofei Zheng,Huajun Wang

Evidence-based complementary and alternative medicine : eCAM 2022:4933255 PubMed35733628

2022

Therapeutic Effect of Astragali Radix Extract Injection Combined with Bone Marrow Mesenchymal Stem Cells in Bleomycin-Induced Pulmonary Fibrotic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Quanyu Du,Xuanyu Wu,Liping An,Rui Zhou,Xiang Xiao,Cheng Wu,Fei Wang,Han Yang

Stem cell research & therapy 13:80 PubMed35197108

2022

Exosomes from tendon derived stem cells promote tendon repair through miR-144-3p-regulated tenocyte proliferation and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Song,Tao Jiang,Pin Pan,Yao Yao,Qing Jiang

Genomics 113:3128-3140 PubMed34245829

2021

The molecular mechanisms of oxygen-sensing in human ductus arteriosus smooth muscle cells: A comprehensive transcriptome profile reveals a central role for mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel E T Bentley,Charles C T Hindmarch,Kimberly J Dunham-Snary,Brooke Snetsinger,Jeffrey D Mewburn,Arthur Thébaud,Patricia D A Lima,Bernard Thébaud,Stephen L Archer

Aging 13:3045-3059 PubMed33460398

2021

Transplantation of bone marrow-derived mesenchymal stem cells with silencing of microRNA-138 relieves pelvic organ prolapse through the FBLN5/IL-1β/elastin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Zhao,Qing Sun,Yazhou Fan,Xinming Hu,Linyu Li,Junmin Wang,Shihong Cui

Journal of orthopaedic surgery and research 16:43 PubMed33430899

2021

Mesenchymal stem cells genetically engineered to express platelet-derived growth factor and heme oxygenase-1 ameliorate osteoarthritis in a canine model.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwon Oh,Yeon Sung Son,Wan Hee Kim,Oh-Kyeong Kwon,Byung-Jae Kang

International journal of nanomedicine 14:5831-5848 PubMed31534327

2019

In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications.

Applications

Unspecified application

Species

Unspecified reactive species

Yucheng Lin,Lu Zhang,Nancy Q Liu,Qingqiang Yao,Ben Van Handel,Yan Xu,Chen Wang,Denis Evseenko,Liming Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD90 / Thy1 antibody [MRC OX-7]

Flow Cytometry - PE Anti-CD90 / Thy1 antibody [MRC OX-7] (AB33694)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise