Rabbit Recombinant Monoclonal p16INK4A antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Images
![Flow Cytometry (Intracellular) - PE Anti-CDKN2A/p16INK4a antibody [EPR1473] (AB209579), expandable thumbnail](https://content.abcam.com/products/images/pe-cdkn2a-p16ink4a-antibody-epr1473-ab209579--flow-cytometry-intracellular-img26137.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - PE Anti-CDKN2A/p16INK4a antibody [EPR1473] (AB209579)")
Publications
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- Biogerontology 21:773-7862020Multiparameter flow cytometric detection and quantification of senescent cells in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdeolu Badi Adewoye,Dimitris Tampakis,Antonia Follenzi,Alexandra StolzingPubMed 32776262
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | |
|---|---|---|
| Human | Not recommended | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min) |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478 - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
4 products for Alternative Product
2 products for Alternative Version
Target data
Function
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Alternative names
Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, p16-INK4a, CDK4I, MTS-1, p16-INK4, p16INK4A, CDKN2A, MTS1, CDKN2
Recommended products
Rabbit Recombinant Monoclonal p16INK4A antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR1473
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Do Not Freeze, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - PE Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab209579), expandable thumbnail](https://content.abcam.com/products/images/pe-cdkn2a-p16ink4a-antibody-epr1473-ab209579--flow-cytometry-intracellular-img26137.jpg "Flow Cytometry (Intracellular) - PE Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab209579)")
Flow Cytometry (Intracellular) - PE Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab209579)
Overlay histogram showing HEK293 cells stained with ab209579 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209579, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HEK293 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
Downloads
Product protocols
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