AB224000

PE Anti-Choline Acetyltransferase antibody [EPR13024(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Choline Acetyltransferase antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT

1 Images

Flow Cytometry (Intracellular) - PE Anti-Choline Acetyltransferase antibody [EPR13024(B)] (AB224000)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Choline Acetyltransferase antibody [EPR13024(B)] (AB224000)

Overlay histogram showing SHSY5Y cells stained with ab224000 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224000, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

660 APC

APC Anti-Choline Acetyltransferase antibody [EPR13024(B)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Choline Acetyltransferase antibody [EPR13024(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Choline Acetyltransferase antibody [EPR13024(B)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Choline Acetyltransferase antibody [EPR13024(B)]
Unconjugated

Anti-Choline Acetyltransferase antibody [EPR13024(B)]
Carrier free

Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13024(B)

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.

Biological function summary

The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.

Pathways

Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.

Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

See full target information CHAT

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1482-1495 PubMed40921734

2025

Hbo1 and Msl complexes preserve differential compaction and H3K27me3 marking of active and inactive X chromosomes during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dounia Djeghloul,Sherry Cheriyamkunnel,Bhavik Patel,Holger Kramer,Alex Montoya,Karen E Brown,Chad Whilding,Tatyana B Nesterova,Guifeng Wei,Neil Brockdorff,Iga Grządzielewska,Remzi Karayol,Asifa Akhtar,Matthias Merkenschlager,Amanda G Fisher

Nature immunology 23:605-618 PubMed35352063

2022

B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian J Schloss,Maarten Hulsmans,David Rohde,I-Hsiu Lee,Nicolas Severe,Brody H Foy,Fadi E Pulous,Shuang Zhang,Konstantinos D Kokkaliaris,Vanessa Frodermann,Gabriel Courties,Chongbo Yang,Yoshiko Iwamoto,Anders Steen Knudsen,Cameron S McAlpine,Masahiro Yamazoe,Stephen P Schmidt,Gregory R Wojtkiewicz,Gustavo Santos Masson,Karin Gustafsson,Diane Capen,Dennis Brown,John M Higgins,David T Scadden,Peter Libby,Filip K Swirski,Kamila Naxerova,Matthias Nahrendorf

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-Choline Acetyltransferase antibody [EPR13024(B)]

Flow Cytometry (Intracellular) - PE Anti-Choline Acetyltransferase antibody [EPR13024(B)] (AB224000)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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