AB209581

PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal COX1 / Cyclooxygenase 1 antibody - conjugated to PE. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

COX1, PTGS1, Prostaglandin G/H synthase 1, Cyclooxygenase-1, Prostaglandin H2 synthase 1, Prostaglandin-endoperoxide synthase 1, COX-1, PGH synthase 1, PGHS-1, PHS 1

1 Images

Flow Cytometry (Intracellular) - PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (AB209581)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (AB209581)

Overlay histogram showing HeLa cells stained with ab209581 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209581, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 585/40 bandpass filter.

Carrier free

Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free
Unconjugated

Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]
HRP

HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5866

Isotype

IgG

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cyclooxygenase 1 (COX-1) also known as COX1 cyclooxygenase-1 COX one and the COX1 protein is an important enzyme in the conversion of arachidonic acid to prostaglandins. It is a heme-containing dimer enzyme with a molecular weight of approximately 70 kDa. COX-1 is widely expressed in most tissues and cell types where it plays a significant role in maintaining homeostatic functions. This protein is constitutively active meaning it is often active under normal physiological conditions.

Biological function summary

COX-1 is involved in producing prostaglandins that regulate a variety of normal physiological processes including gastric mucosal protection renal blood flow and platelet aggregation. Unlike COX-2 COX-1 is not induced by inflammatory stimuli and is not part of an inducible complex. It serves to maintain essential physiological functions in various organs and systems making its activity critical for cellular maintenance.

Pathways

COX-1 is primarily involved in the prostaglandin biosynthesis pathway. It converts arachidonic acid into prostaglandin H2 a precursor for other prostaglandins and thromboxanes. Thromboxane A2 produced from this pathway plays an important role in platelet aggregation and vasoconstriction linking COX-1's functions with hemostatic processes. Another protein involved in this pathway is thromboxane synthase which further processes the products of COX-1 activity.

COX-1’s role connects it closely to conditions like peptic ulcers and cardiovascular diseases. Inhibition of COX-1 by nonsteroidal anti-inflammatory drugs (NSAIDs) can lead to gastric mucosal damage contributing to the development of peptic ulcers. Additionally due to its involvement in platelet aggregation COX-1 affects thrombotic diseases. COX-1's interactions with proteins such as COX-2 become relevant in inflammation and pain management where selective inhibition of COX-2 is sought to reduce adverse effects related to COX-1.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Dual cyclooxygenase and peroxidase that plays an important role in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20 : 4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (PubMed : 7947975). Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells (Probable). Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18 : 2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products (By similarity).

See full target information PTGS1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biogerontology 21:773-786 PubMed32776262

2020

Multiparameter flow cytometric detection and quantification of senescent cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Adeolu Badi Adewoye,Dimitris Tampakis,Antonia Follenzi,Alexandra Stolzing

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]

Flow Cytometry (Intracellular) - PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (AB209581)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise