JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326180

PE Anti-CPOX antibody [EPR11927(B)]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal CPOX antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CPO, CPX, CPOX, COX, Coprogen oxidase, Coproporphyrinogenase

1 Images
Flow Cytometry (Intracellular) - PE Anti-CPOX antibody [EPR11927(B)] (AB326180)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-CPOX antibody [EPR11927(B)] (AB326180)

Flow cytometry scatter plots showing human peripheral mononuclear cells (PBMCs), either stained with ab326180 (right) or isotype control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab326180) (1x 106 in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice. Isotype control antibody was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). The cells were simultaneously stained with CD3. Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11927(B)

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPOX or coproporphyrinogen oxidase catalyzes the oxidation of coproporphyrinogen III to protoporphyrinogen IX an important step in heme biosynthesis. CPOX possesses a molecular mass of approximately 40 kDa. Expression occurs primarily in the mitochondria of liver and bone marrow cells. This enzyme plays a role in maintaining the balance of porphyrin intermediates and prevents accumulation that may cause cellular toxicity.
Biological function summary

CPOX converts coproporphyrinogen III into protoporphyrinogen IX playing a role in the heme biosynthetic pathway. As part of the terminal steps in heme production this transformation is important for synthesizing heme an essential component of hemoglobin myoglobin and various cytochromes. CPOX is not part of any larger enzyme complex but interacts with adjacent enzymes in the metabolic pathway to ensure efficient heme synthesis.

Pathways

CPOX functions in the heme biosynthesis pathway. This pathway is vital for the production of heme enabling the synthesis of hemoglobin and electron transport chains. CPOX acts sequentially after coproporphyrinogen III oxidase and before protoporphyrinogen IX oxidase. The enzyme works in concert with proteins such as uroporphyrinogen III synthase (UROS) in earlier steps of the pathway affecting overall heme production efficiency.

Mutations or defects in CPOX can lead to hereditary coproporphyria (HCP) a disorder characterized by neurovisceral attacks and photosensitivity. This condition results from the accumulation of porphyrins due to a dysfunctional heme synthesis pathway. Additionally CPOX-related dysfunction can have implications in liver disorders affecting the balance of heme precursors. Improved understanding of CPOX and its interactions with proteins like UROS can provide insight into therapeutic targets for these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX and participates to the sixth step in the heme biosynthetic pathway.
See full target information CPOX
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com