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AB326527

PE Anti-CTLA4 antibody [BNI3]

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Mouse Monoclonal CTLA4 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD152, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4

3 Images
Flow Cytometry - PE Anti-CTLA4 antibody [BNI3] (AB326527)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CTLA4 antibody [BNI3] (AB326527)

Flow cytometry multicolor surface staining of human PHA stimulated lymphocytes stained using ab326527 (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - PE Anti-CTLA4 antibody [BNI3] (AB326527)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CTLA4 antibody [BNI3] (AB326527)

Flow cytometry surface staining pattern of human PHA stimulated peripheral whole blood stained using ab326527 (10 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - PE Anti-CTLA4 antibody [BNI3] (AB326527)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CTLA4 antibody [BNI3] (AB326527)

Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral whole blood stained using ab326527 (10 μl reagent / 100 μl of peripheral whole blood).

  • 660 APC

    APC Anti-CTLA4 antibody [BNI3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BNI3

Isotype

IgG2a

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The mouse monoclonal antibody BNI3 recognizes an extracellular domain of human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitory receptor acting as a major negative regulator of T-cell responses (PubMed : 11279501, PubMed : 11279502, PubMed : 16551244, PubMed : 1714933, PubMed : 18641304, PubMed : 28484017). Acts as a decoy receptor : the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (PubMed : 11279501, PubMed : 11279502, PubMed : 16551244, PubMed : 1714933, PubMed : 28484017).
See full target information CTLA4
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Product promise

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