AB157301

PE/Cy5® Anti-c-Kit antibody [104D2]

Name: PE/Cy5® Anti-c-Kit antibody [104D2] (ab157301) | Abcam
Brand: Abcam Limited
SKU: AB157301
Price: 220 USD
Availability: InStock

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(5 Publications)

Mouse Monoclonal c-Kit antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Human, Primates, Cow samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing KIT protein.

View Alternative Names

CD117, SCFR, KIT, Mast/stem cell growth factor receptor Kit, Piebald trait protein, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, PBT

3 Images

Flow Cytometry - PE/Cy5® Anti-c-Kit antibody [104D2] (AB157301)

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-c-Kit antibody [104D2] (AB157301)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab157301 (4 μl reagent / 100 μl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-c-Kit antibody [104D2] (AB157301)

Separation of human CD34 positive stem cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab157301 (4 μl reagent / 100 μl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-c-Kit antibody [104D2] (AB157301)

Flow cytometry surface staining pattern of human peripheral whole blood showing CD34 positive stem cells (red) stained using ab157301 (4 μl reagent / 100 μl of peripheral whole blood).

660 APC

APC Anti-c-Kit antibody [104D2]
785 APC/Cy7®

APC/Cy7® Anti-c-Kit antibody [104D2]
Biotin

Biotin Anti-c-Kit antibody [104D2]
519 FITC

FITC Anti-c-Kit antibody [104D2]
578 PE

PE Anti-c-Kit antibody [104D2]
778 PE/Cy7®

PE/Cy7® Anti-c-Kit antibody [104D2]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-c-Kit antibody [104D2]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

104D2

Isotype

IgG1

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

Reacts with

Cow, Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing KIT protein. The exact immunogen used to generate this antibody is proprietary information.

P10721

Specificity

ab157301 detects the extracellular part of c-Kit.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "Use 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. <a href='/en-us/products/primary-antibodies/pe-cy5-mouse-igg1-kappa-monoclonal-15h6-isotype-control-ab67435'>ab67435</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "(or 100 ul of whole blood). <a href='/en-us/products/primary-antibodies/pe-cy5-mouse-igg1-kappa-monoclonal-15h6-isotype-control-ab67435'>ab67435</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Use 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. b67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Product details

Purified antibody is conjugated with tandem dye PE/Cy5^® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Kit protein also known as CD117 is a type of receptor tyrosine kinase with a molecular weight of approximately 145 kDa. It is encoded by the KIT gene and commonly expressed on the surface of hematopoietic stem cells mast cells melanocytes and germ cells. C-Kit plays a role in the signal transduction pathways that control cell proliferation and survival. Researchers often identify c-Kit using markers like biotin c-Kit PerCP Cy5.5 or antibodies such as anti-c-Kit for experimental purposes.

Biological function summary

C-Kit influences the function and survival of certain cell types. When it binds to its ligand stem cell factor (SCF) it activates receptor dimerization and phosphorylation triggering downstream signaling. C-Kit does not act alone; it often functions in a complex with other proteins to affect cellular outcomes. Its expression is vital for the maintenance of bone marrow hematopoietic cells and contributes to melanocyte and germ cell development.

Pathways

C-Kit interacts with the PI3K/AKT and RAS/ERK pathways through its kinase activity. These pathways play important roles in cell division differentiation and survival. The c-Abl and ACK2 proteins are closely related to c-Kit function within these signaling networks contributing to regulating cellular responses to external stimuli.

C-Kit mutations are frequently associated with gastrointestinal stromal tumors (GIST) and acute myeloid leukemia (AML). These conditions often involve alterations in c-Kit activity leading to uncontrolled cell growth. While studying GIST researchers have noticed interactions between c-Kit and other proteins like CBL which is involved in the modulation of c-Kit signaling. Understanding these interactions helps in designing targeted therapies for conditions involving c-Kit dysregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

See full target information KIT

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38334641

2024

Gestational Intermittent Hypoxia Enhances Mammary Stem Cells and Alters Tumor Phenotype in Adult Female Offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Jaitri Joshi,Yue Xiong,Molly Kuhn,Abigail B Radcliff,Tracy L Baker,Jyoti J Watters,Lisa M Arendt

American journal of translational research 10:2511-2528 PubMed30210689

2018

Transcription factor Tbx18 induces the differentiation of c-kit canine mesenchymal stem cells (cMSCs) into SAN-like pacemaker cells in a co-culture model in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Xiao,Yong-Jun Yang,Yi-Zhang Lin,Song Peng,Shu Lin,Zhi-Yuan Song

Blood 94:1979-86 PubMed10477727

1999

Signaling via Src family kinases is required for normal internalization of the receptor c-Kit.

Applications

Unspecified application

Species

Unspecified reactive species

V C Broudy,N L Lin,W C Liles,S J Corey,B O'Laughlin,S Mou,D Linnekin

Blood 91:898-906 PubMed9446650

1998

Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer.

Applications

Unspecified application

Species

Unspecified reactive species

V C Broudy,N L Lin,H J Bühring,N Komatsu,T J Kavanagh

Blood 90:111-25 PubMed9207445

1997

Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase.

Applications

Unspecified application

Species

Unspecified reactive species

I Rappold,B L Ziegler,I Köhler,S Marchetto,O Rosnet,D Birnbaum,P J Simmons,A C Zannettino,B Hill,S Neu,W Knapp,R Alitalo,K Alitalo,A Ullrich,L Kanz,H J Bühring

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE/Cy5® Anti-c-Kit antibody [104D2]

Flow Cytometry - PE/Cy5® Anti-c-Kit antibody [104D2] (AB157301)

Flow Cytometry - PE/Cy5® Anti-c-Kit antibody [104D2] (AB157301)

Flow Cytometry - PE/Cy5® Anti-c-Kit antibody [104D2] (AB157301)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise