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Rat Monoclonal CD11b antibody - conjugated to Cy5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing Itgam protein.


Images

Key facts

Isotype

IgG2b

Host species

Rat

Conjugation

Cy5®

Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.14% Monobasic dihydrogen sodium phosphate, 0.1% Gelatin

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing Itgam protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05555
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (PubMed:18685038).

Alternative names

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Rat Monoclonal CD11b antibody - conjugated to Cy5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing Itgam protein.

Alternative names

Key facts

Isotype

IgG2b

Conjugation

Cy5®

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing Itgam protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05555
Clone number

M1/70

Purification technique

Affinity purification

Light chain type

kappa

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

Supplementary info

Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Activity summary

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

Associated diseases and disorders

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.

Product promise

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4 product images

  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533), expandable thumbnail

    Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

    Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab25533 at 0.125 μg (solid line) or Rat IgG2b (PE/Cy5®) isotype control at 0.125 μg (dashed line).

  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

    ab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy: Neutrophils.

  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533), expandable thumbnail

    Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

    Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions.

  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

    ab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.

    Gating Strategy: Live Macrophages.

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Product protocols

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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