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AB25533

PE/Cy5® Anti-CD11b antibody [M1/70]

4

(13 Reviews)

|

(11 Publications)

Rat Monoclonal CD11b antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 11 publications. Immunogen corresponding to Cell preparation containing Itgam protein.

View Alternative Names

CD11b, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Itgam

4 Images
Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)
  • Flow Cyt

AbReview19983****

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)

ab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy : Neutrophils.

This image is courtesy of an anonymous Abreview

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)

Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab25533 at 0.125 μg (solid line) or Rat IgG2b (PE/Cy5®) isotype control at 0.125 μg (dashed line).

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)
  • Flow Cyt

Unknown

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)

Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions.

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)
  • Flow Cyt

AbReview28847****

Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (AB25533)

ab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Gating Strategy : Live Macrophages.

This image is courtesy of an anonymous Abreview

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11b antibody [M1/70]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11b antibody [M1/70]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

  • Unconjugated

    Anti-CD11b antibody [M1/70]

  • Carrier free

    Anti-CD11b antibody [M1/70] - BSA and Azide free

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD11b antibody [M1/70]

  • 660 APC

    APC Anti-CD11b antibody [M1/70]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD11b antibody [M1/70]

  • redFluor™ 710

    redFluor™ 710 Anti-CD11b antibody [M1/70]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD11b antibody [M1/70]

  • Biotin

    Biotin Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

  • 519 FITC

    FITC Anti-CD11b antibody [M1/70]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD11b antibody [M1/70]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M1/70

Isotype

IgG2b

Light chain type

kappa

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Itgam protein. The exact immunogen used to generate this antibody is proprietary information.

P05555

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (PubMed : 18685038).
See full target information Itgam
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36835513

2023

Tissue-Oxygen-Adaptation of Bone Marrow-Derived Mesenchymal Stromal Cells Enhances Their Immunomodulatory and Pro-Angiogenic Capacity, Resulting in Accelerated Healing of Chemical Burns.

Applications

Unspecified application

Species

Unspecified reactive species

Marina V Volkova,Ningfei Shen,Anna Polyanskaya,Xiaoli Qi,Valery V Boyarintsev,Elena V Kovaleva,Alexander V Trofimenko,Gleb I Filkov,Alexandre V Mezentsev,Sergey P Rybalkin,Mikhail O Durymanov

Frontiers in immunology 13:941219 PubMed35911675

2022

Cold Plasma Irradiation Attenuates Atopic Dermatitis Enhancing HIF-1α-Induced MANF Transcription Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Sun,Xinru Zhang,Chao Hou,Shujun Yu,Yujing Zhang,Zhuo Yu,Ling Kong,Changqing Liu,Lijie Feng,Dong Wang,Guohua Ni

Bioactive materials 6:2029-2038 PubMed33474514

2021

Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohan Dai,Boon Chin Heng,Yunyang Bai,Fuping You,Xiaowen Sun,Yiping Li,Zhangui Tang,Mingming Xu,Xuehui Zhang,Xuliang Deng

Redox biology 34:101560 PubMed32413745

2020

Melatonin alleviates morphine analgesic tolerance in mice by decreasing NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qianjin Liu,Ling-Yan Su,Chunli Sun,Lijin Jiao,Ying Miao,Min Xu,Rongcan Luo,Xin Zuo,Rongbin Zhou,Ping Zheng,Wei Xiong,Tian Xue,Yong-Gang Yao

Acta biomaterialia 72:434-446 PubMed29649639

2018

Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation of RhoA expression.

Applications

Flow Cyt

Species

Unspecified reactive species

Jing Xu,Junyao Yang,Agata Nyga,Mazdak Ehteramyan,Ana Moraga,Yuanhao Wu,Lingfang Zeng,Martin M Knight,Julia C Shelton

Journal of the American Heart Association 7: PubMed29437605

2018

Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Guanmei Wen,Weiwei An,Jiangyong Chen,Eithne M Maguire,Qishan Chen,Feng Yang,Stuart W A Pearce,Maria Kyriakides,Li Zhang,Shu Ye,Sussan Nourshargh,Qingzhong Xiao

The Journal of biological chemistry 292:14885-14901 PubMed28724635

2017

Heterodimers of the transcriptional factors NFATc3 and FosB mediate tissue factor expression for 15()-hydroxyeicosatetraenoic acid-induced monocyte trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Sivareddy Kotla,Nikhlesh K Singh,Daniel Kirchhofer,Gadiparthi N Rao

Scientific reports 6:24152 PubMed27080036

2016

Retinoic acid facilitates inactivated transmissible gastroenteritis virus induction of CD8(+) T-cell migration to the porcine gut.

Applications

Flow Cyt

Species

Unspecified reactive species

Xiaojuan Chen,Chongzhi Tu,Tao Qin,Liqi Zhu,Yinyan Yin,Qian Yang

Molecular medicine reports 12:2511-20 PubMed25936350

2015

Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Lin Zeng,Li-Hui Wang,Ping Li,Wei Wang,Jiong Yang

Blood 124:1987-95 PubMed24869940

2014

Stable long-term mixed chimerism achieved in a canine model of allogeneic in utero hematopoietic cell transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse D Vrecenak,Erik G Pearson,Matthew T Santore,Carlyn A Todorow,Haiying Li,Antoneta Radu,Tricia Bhatti,William H Peranteau,Mark P Johnson,Alan W Flake
View all publications
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