AB25508

PE/Cy5® Anti-CD19 antibody [6D5]

Lab Essentials
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(3 Publications)

Rat Monoclonal CD19 antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing Cd19 protein.

View Alternative Names

CD19, B-lymphocyte antigen CD19, Differentiation antigen CD19, Cd19

1 Images

Flow Cytometry - PE/Cy5® Anti-CD19 antibody [6D5] (AB25508)

Flow Cyt

Unknown

Flow Cytometry - PE/Cy5® Anti-CD19 antibody [6D5] (AB25508)

Flow cytometric analysis of C57BL/6 mouse splenocytes labelling CD19 with ab25508 at 0.01 μg/10⁶ cells and labelling CD4 with a Rat Anti-Mouse CD4-PE antibody (ab25496).

Unconjugated

Anti-CD19 antibody [6D5]
695 PE/Cy5.5®

PE/Cy5.5® Anti-CD19 antibody [6D5]
667 Cy5®

Cy5 ® Anti-CD19 antibody [6D5]
660 APC

APC Anti-CD19 antibody [6D5]
778 PE/Cy7®

PE/Cy7® Anti-CD19 antibody [6D5]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

6D5

Isotype

IgG2a

Light chain type

kappa

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd19 protein. The exact immunogen used to generate this antibody is proprietary information.

P25918

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.1 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose, 0.2% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (By similarity). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 20101619, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 7542548, PubMed : 7543183, PubMed : 9317126). Required for normal differentiation of B-1 cells (PubMed : 12387743, PubMed : 7542548, PubMed : 7543183). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 12387743, PubMed : 7542548, PubMed : 9317126). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 7542548, PubMed : 7543183).

See full target information Cd19

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 157:48-56 PubMed8683154

1996

The signaling activity of murine CD19 is regulated during cell development.

Applications

Unspecified application

Species

Unspecified reactive species

I Krop,A L Shaffer,D T Fearon,M S Schlissel

European journal of immunology 26:238-42 PubMed8566073

1996

Self-renewal of B-1 lymphocytes is dependent on CD19.

Applications

Unspecified application

Species

Unspecified reactive species

I Krop,A R de Fougerolles,R R Hardy,M Allison,M S Schlissel,D T Fearon

Current opinion in immunology 5:341-8 PubMed7688513

1993

The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

D T Fearon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE/Cy5® Anti-CD19 antibody [6D5]

Flow Cytometry - PE/Cy5® Anti-CD19 antibody [6D5] (AB25508)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise