Mouse Monoclonal CD3d antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing CD3D protein.
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![Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1] (AB157300), expandable thumbnail](https://content.abcam.com/products/images/pe-cy5-cd3-antibody-ucht1-ab157300--flow-cytometry-img75975.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1] (AB157300)")
Publications
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- International archives of allergy and immunology 184:85-972022Changes of T Lymphocyte Subsets in Peripheral Blood of Patients with Intermediate and Advanced Cervical Cancer before and after Nimotuzumab Combined with Chemoradiotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Teng,Guimin Cui,Ligang Qian,Lujun ZhaoPubMed 36265457
- Oncology letters 12:4087-40932016Establishment of specific cytotoxic T lymphocyte culture system and its inhibitory effect on ovarian cancer.Applications:Flow CytReactive species:HumanMingxing Sui,Lihui Si,Tianmin Xu,Manhua CuiPubMed 27895776
- The Journal of experimental medicine 204:2537-442007Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabrielle M Siegers et. al.PubMed 17923503
- The New England journal of medicine 354:1913-212006Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrédéric Rieux-Laucat,Claire Hivroz,Annick Lim,Véronique Mateo,Isabelle Pellier,Françoise Selz,Alain Fischer,Françoise Le DeistPubMed 16672702
- Proceedings of the National Academy of Sciences of 101:16268-732004Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelly L Arnett et. al.PubMed 15534202
- Journal of immunology (Baltimore, Md. : 1950) 161:1113-221998Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS le Gouvello,V Manceau,A SobelPubMed 9686569
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE/Cy5®
- Excitation/Emission
- Ex: 496nm, Em: 667nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04234
Reactivity data
Select an application| Flow Cyt | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Expected |
| Rat | Expected | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µL for 106 Cells | Notes (or 100 μl of whole blood). ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 4 µL for 106 Cells | Notes (or 100 μl of whole blood). ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µL for 106 Cells | Notes (or 100 μl of whole blood). ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info 4 µL for 106 Cells | Notes (or 100 μl of whole blood). ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
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Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).
Alternative names
CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain
Recommended products
Mouse Monoclonal CD3d antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing CD3D protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE/Cy5®
- Excitation/Emission
- Ex: 496nm, Em: 667nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04234
- Clone number
- UCHT1
- Purification technique
- Size-exclusion chromatography
- Epitope
- ab157300 reacts with the epsilon chain of the CD3 complex.
- Concentration
- Purification notes
Purified antibody is conjugated with tandem dye PE/Cy5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1] (ab157300), expandable thumbnail](https://content.abcam.com/products/images/pe-cy5-cd3-antibody-ucht1-ab157300--flow-cytometry-img75975.jpg "Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1] (ab157300)")
Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1] (ab157300)
CD3 Flow Cytometry staining using mouse Anti-CD3 antibody
Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) PE-CyTM5.
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