AB25531

PE/Cy5® Anti-CD3 epsilon antibody [C363.29B]

Name: PE/Cy5® Anti-CD3 epsilon antibody [C363.29B] (ab25531) | Abcam
Brand: Abcam Limited
SKU: AB25531
Price: 225 USD
Availability: InStock

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(2 Publications)

Rat Monoclonal CD3E antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e

1 Images

Flow Cytometry - PE/Cy5® Anti-CD3 epsilon antibody [C363.29B] (AB25531)

Flow Cyt

Unknown

Flow Cytometry - PE/Cy5® Anti-CD3 epsilon antibody [C363.29B] (AB25531)

Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (PE/Cy5) ab25531 (1 µg/10⁶cells) and Rat Anti-Mouse CD19-PE.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

C363.29B

Isotype

IgG2c

Light chain type

kappa

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Epitope

This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.1 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose, 0.2% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed : 19956738, PubMed : 24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).

See full target information Cd3e

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70161 PubMed39815665

2025

A Risk Model Based on Ferroptosis-Related Genes OSMR, G0S2, IGFBP6, IGHG2, and FMOD Predicts Prognosis in Glioblastoma Multiforme.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqiu Wu,Ling Liu,Zhili Li,Tian Zhang,Qi Wang,Meixiong Cheng

Brain : a journal of neurology 123 ( Pt 6):1092-101 PubMed10825349

2000

Differential adhesion molecule requirements for immune surveillance and inflammatory recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

M D Carrithers,I Visintin,S J Kang,C A Janeway

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE/Cy5® Anti-CD3 epsilon antibody [C363.29B]

Flow Cytometry - PE/Cy5® Anti-CD3 epsilon antibody [C363.29B] (AB25531)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Epitope

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Product promise