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AB169823

PE/Cy5® Anti-CD33 antibody [HIM3-4]

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(3 Publications)

Mouse Monoclonal CD33 antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3

1 Images
Flow Cytometry - PE/Cy5® Anti-CD33 antibody [HIM3-4] (AB169823)
  • Flow Cyt

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Flow Cytometry - PE/Cy5® Anti-CD33 antibody [HIM3-4] (AB169823)

Flow cytometric analysis of normal human peripheral blood cells labeling CD33 with ab169823 at 5μl/105-106 cells (filled histogram) or PE-Cy5 Mouse IgG1 K Isotype Control (open histogram). Cells in the monocyte gate were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HIM3-4

Isotype

IgG1

Light chain type

kappa

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-cy5-mouse-igg1-kappa-monoclonal-15h6-isotype-control-ab67435'>ab67435</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed : 10611343, PubMed : 11320212, PubMed : 15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed : 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed : 10887109, PubMed : 28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 10206955, PubMed : 10556798, PubMed : 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed : 10206955, PubMed : 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed : 15597323).
See full target information CD33
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 21:e1011600 PubMed40043053

2025

Human CD33 deficiency is associated with mild alteration of circulating white blood cell counts.

Applications

Unspecified application

Species

Unspecified reactive species

John Dominy,Jirong Bai,Christopher Koch,Maleeha Zaman Khan,Shareef Khalid,Jonathan H Chung,Madhura Panditrao,Lulu Liu,Qi Zhang,Muhammad Jahanzaib,Muhammad Rehan Mian,Muhammad Bilal Liaqat,Syed Shahzaib Raza,Riffat Sultana,Anjum Jalal,Muhammad Hamid Saeed,Shahid Abbas,Fazal Rehman Memon,Mohammad Ishaq,Kashif Saleheen,Asif Rasheed,Allan Gurtan,Danish Saleheen

Emerging microbes & infections 13:2387448 PubMed39109538

2024

TCR-like bispecific antibodies toward eliminating infected hepatocytes in HBV mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Shi,Zihan Wang,Jingjing Xu,Wenxia Niu,Yubin Wu,Huiyu Guo,Jinmiao Shi,Zonglin Li,Baorong Fu,Yunda Hong,Zikang Wang,Wenjie Guo,Dabing Chen,Xingling Li,Qian Li,Shaojuan Wang,Jiahua Gao,Aling Sun,Yaosheng Xiao,Jiali Cao,Lijuan Fu,Yangtao Wu,Tianying Zhang,Ningshao Xia,Quan Yuan

F1000Research 12:172 PubMed38106655

2023

Identification of high-performing antibodies for Moesin for use in Western Blot, immunoprecipitation, and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Walaa Alshafie,Riham Ayoubi,Maryam Fotouhi,Kathleen Southern,Carl Laflamme
View all publications
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Product promise

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