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AB233448

PE/Cy5® Anti-CD33 antibody [WM53]

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(1 Publication)

Mouse Monoclonal CD33 antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD33 protein.

View Alternative Names

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3

3 Images
Flow Cytometry - PE/Cy5® Anti-CD33 antibody [WM53] (AB233448)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD33 antibody [WM53] (AB233448)

Surface staining of human peripheral blood cells with ab233448 (4 μl reagent / 100 μl of whole blood). Gated on leukocytes.

Flow Cytometry - PE/Cy5® Anti-CD33 antibody [WM53] (AB233448)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD33 antibody [WM53] (AB233448)

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab233448 (4 µl reagent / 100 µl of peripheral whole blood).

Flow Cytometry - PE/Cy5® Anti-CD33 antibody [WM53] (AB233448)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD33 antibody [WM53] (AB233448)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab233448 (4 µl reagent / 100 µl of peripheral whole blood).

  • Unconjugated

    Anti-CD33 antibody [WM53]

  • Carrier free

    Anti-CD33 antibody [WM53] - BSA and Azide free

  • 660 APC

    APC Anti-CD33 antibody [WM53]

  • 675 PerCP

    PerCP Anti-CD33 antibody [WM53]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD33 antibody [WM53]

  • 578 PE

    PE Anti-CD33 antibody [WM53]

  • 519 FITC

    FITC Anti-CD33 antibody [WM53]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD33 antibody [WM53]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD33 antibody [WM53]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

WM53

Isotype

IgG1

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD33 protein. The exact immunogen used to generate this antibody is proprietary information.

P20138

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed : 10611343, PubMed : 11320212, PubMed : 15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed : 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed : 10887109, PubMed : 28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 10206955, PubMed : 10556798, PubMed : 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed : 10206955, PubMed : 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed : 15597323).
See full target information CD33
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 2: PubMed29263297

2017

Human pericytes adopt myofibroblast properties in the microenvironment of the IPF lung.

Applications

Unspecified application

Species

Unspecified reactive species

Parid Sava,Anand Ramanathan,Amelia Dobronyi,Xueyan Peng,Huanxing Sun,Adrian Ledesma-Mendoza,Erica L Herzog,Anjelica L Gonzalez
View all publications
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Product promise

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