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AB25476

PE/Cy5® Anti-CD4 antibody [GK1.5]

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(1 Publication)

Rat Monoclonal CD4 antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4

1 Images
Flow Cytometry - PE/Cy5® Anti-CD4 antibody [GK1.5] (AB25476)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD4 antibody [GK1.5] (AB25476)

Flow cytometry analysis showing C57Bl/6 splenocytes stained with ab25476 (sold line). The isotype control was PE-Cy5 Rat IgG2b.

  • 660 APC

    APC Anti-CD4 antibody [GK1.5]

  • 519 FITC

    FITC Anti-CD4 antibody [GK1.5]

  • 519 FITC

    FITC Anti-CD4 antibody [GK1.5]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD4 antibody [GK1.5]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD4 antibody [GK1.5]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD4 antibody [GK1.5]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

GK1.5

Isotype

IgG2b

Light chain type

kappa

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
See full target information Cd4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23443 PubMed38265281

2024

Interleukin 28A aggravates Con A-induced acute liver injury by promoting the recruitment of M1 macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Zhang,Dalei Cheng,Hui Zhang,Zhihong Liu,Min Gao,Li Wei,Fenglian Yan,Chunxia Li,Lin Wang,Guanjun Dong,Changying Wang,Mingsheng Zhao,Yuanbo Zhu,Huabao Xiong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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