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AB25044

PE/Cy5® Anti-CD40 antibody [1C10]

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(3 Publications)

Rat Monoclonal CD40 antibody - conjugated to PE/Cy5®. Suitable for IP, Flow Cyt, FuncS and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD40.

View Alternative Names

CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40

1 Images
Flow Cytometry - PE/Cy5® Anti-CD40 antibody [1C10] (AB25044)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD40 antibody [1C10] (AB25044)

BALB/c mouse splenocytes were stained with Rat Anti- Mouse CD40-PE/Cy5 (ab25044). The grey peak represents an isotype control. The purple peaks represents the negative (Left) and positive (right) datasets.

  • Unconjugated

    Anti-CD40 antibody [1C10]

  • 660 APC

    APC Anti-CD40 antibody [1C10]

  • 578 PE

    PE Anti-CD40 antibody [1C10]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

1C10

Isotype

IgG2a

Light chain type

kappa

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, IP, FuncS

applications

Immunogen

Recombinant Fragment Protein within Human CD40. The exact immunogen used to generate this antibody is proprietary information.

P25942

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>In vitro induction of proliferation of mature B lymphocytes.</p>" } } }
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Product details

This antibody closely resembles the gp39/CD40 ligand in its ability to stimulate proliferation of small, resting B lymphocytes in the absence of other cofactors.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.1 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose, 0.2% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF5/CD40LG (PubMed : 31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
See full target information CD40
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37108601

2023

Extracellular Vesicles Secreted by Pre-Hatching Bovine Embryos Produced In Vitro and In Vivo Alter the Expression of IFNtau-Stimulated Genes in Bovine Endometrial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Constanza Aguilera,Alejandra Estela Velásquez,Miguel Angel Gutierrez-Reinoso,Yat Sen Wong,Barbara Melo-Baez,Joel Cabezas,Diego Caamaño,Felipe Navarrete,Daniela Rojas,Gonzalo Riadi,Fidel Ovidio Castro,Llretny Rodriguez-Alvarez

Frontiers in immunology 12:745939 PubMed34616407

2021

Tumor-Experienced Human NK Cells Express High Levels of PD-L1 and Inhibit CD8 T Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica M Sierra,Florencia Secchiari,Sol Y Nuñez,Ximena L Raffo Iraolagoitia,Andrea Ziblat,Adrián D Friedrich,María V Regge,M Cecilia Santilli,Nicolás I Torres,Mariana Gantov,Aldana Trotta,Carlos Ameri,Gonzalo Vitagliano,Hernando Ríos Pita,Luis Rico,Agustín Rovegno,Nicolás Richards,Carolina I Domaica,Norberto W Zwirner,Mercedes B Fuertes

International journal of molecular sciences 21: PubMed33255183

2020

MicroRNAs from Extracellular Vesicles Secreted by Bovine Embryos as Early Biomarkers of Developmental Competence.

Applications

Unspecified application

Species

Unspecified reactive species

Bárbara Melo-Baez,Yat S Wong,Constanza J Aguilera,Joel Cabezas,Ana C F Mançanares,Gonzalo Riadi,Fidel O Castro,Lleretny Rodriguez-Alvarez
View all publications
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Product promise

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