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AB95520

PE/Cy5® Anti-CD45RO antibody [UCHL1]

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(1 Publication)

Mouse Monoclonal CD45 antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images
Flow Cytometry - PE/Cy5® Anti-CD45RO antibody [UCHL1] (AB95520)
  • Flow Cyt

Unknown

Flow Cytometry - PE/Cy5® Anti-CD45RO antibody [UCHL1] (AB95520)

Flow cytometric staining of normal Human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or ab95520 (filled histogram). Cells in the lymphocyte gate were used for analysis.

  • 660 APC

    APC Anti-CD45R0 antibody [UCHL1]

  • 519 FITC

    FITC Anti-CD45R0 antibody [UCHL1]

  • 578 PE

    PE Anti-CD45R0 antibody [UCHL1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UCHL1

Isotype

IgG2a

Light chain type

kappa

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

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Alternative Products

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45RO also known as leukocyte common antigen 2 is an important isoform of the protein tyrosine phosphatase receptor type C (PTPRC). This protein has a molecular mass of about 180 kDa. Researchers find CD45RO expressed mostly on memory T cells a subset of T lymphocytes. These cells play an essential role in the immune system by remembering past infections or vaccinations. CD45RO functions mechanistically by modulating T cell receptor (TCR) signaling which is important for antibody production.
Biological function summary

This isoform plays an important role in immune responses by supporting the activation and differentiation of T cells. It is not part of a larger protein complex itself but interacts with other immune cell surface proteins for effective signaling. CD45RO is particularly important in the modulation of signal transduction through its phosphatase activity which removes phosphate groups from specific tyrosine residues on proteins influencing T cell sensitivity and response to antigens.

Pathways

CD45RO influences the JAK/STAT pathway a critical signaling pathway for cytokine receptor signaling in immune cells. It also plays a role in the TCR signaling pathway which is responsible for T cell activation. Within these pathways CD45RO acts in conjunction with other proteins like Lck and ZAP-70 that are also integral parts of T cell activation and proliferation processes essential for adaptive immune responses.

CD45RO is associated with autoimmune disorders such as rheumatoid arthritis where memory T cells play a role in sustaining inflammation. CD45RO also relates to chronic hepatitis B infection as it affects the disease progression through memory T cell-mediated immune responses. In these contexts CD45RO's interaction with other proteins like HLA-DR which is another immune-related surface protein becomes significant as it helps modulate immune responses that either suppress or exacerbate the disease symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS medicine 4:e253 PubMed17696642

2007

Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Brigitte Senechal,Gaelle Elain,Eric Jeziorski,Virginie Grondin,Natacha Patey-Mariaud de Serre,Francis Jaubert,Kheira Beldjord,Arielle Lellouch,Christophe Glorion,Michel Zerah,Pierre Mary,Mohammed Barkaoui,Jean Francois Emile,Liliane Boccon-Gibod,Patrice Josset,Marianne Debré,Alain Fischer,Jean Donadieu,Frederic Geissmann
View all publications
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Product promise

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