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Mouse Monoclonal CD62L antibody - conjugated to Cy5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications.


Images

Flow Cytometry - PE/Cy5® Anti-CD62L antibody [DREG-56] (AB95540), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

Cy5®

Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 0.2% BSA

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species

Human

Dilution info

5 µL for 106 Cells

Notes

ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Associated Products

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4 products for Alternative Version

3 products for Alternative Product

Target data

Function

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed:12403782, PubMed:28011641, PubMed:28489325). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed:12403782, PubMed:28011641).

Alternative names

Recommended products

Mouse Monoclonal CD62L antibody - conjugated to Cy5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype

IgG1

Conjugation

Cy5®

Form

Liquid

Clonality

Monoclonal

Clone number

DREG-56

Purification technique

Affinity purification Protein G

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.

Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

Associated diseases and disorders

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.

Product promise

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1 product image

  • Flow Cytometry - PE/Cy5® Anti-CD62L antibody [DREG-56] (ab95540), expandable thumbnail

    Flow Cytometry - PE/Cy5® Anti-CD62L antibody [DREG-56] (ab95540)

    Staining of normal Human peripheral blood cells with PE-Cy5 Mouse IgG1 ? Isotype Control (open histogram) or ab95540 (filled histogram).
    Cells in the lymphocyte gate were used for analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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