Mouse Monoclonal CD63 antibody - conjugated to Cy5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing CD63 protein.
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![Flow Cytometry - PE/Cy5® Anti-CD63 antibody [MEM-259] (AB234251), expandable thumbnail](https://content.abcam.com/products/images/pe-cy5-cd63-antibody-mem-259-ab234251--flow-cytometry-img98412.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE/Cy5® Anti-CD63 antibody [MEM-259] (AB234251)")
Publications
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- Bone & joint research 10:328-3392021Extracellular vesicles derived from mesenchymal stem cells containing microRNA-381 protect against spinal cord injury in a rat model via the BRD4/WNT5A axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXufeng Jia,Guangping Huang,Shaohua Wang,Miao Long,Xiaojun Tang,Daxiong Feng,Qingzhong ZhouPubMed 34024119
- Journal of cellular and molecular medicine 24:9560-95732020Endoplasmic reticulum stress-induced exosomal miR-27a-3p promotes immune escape in breast cancer via regulating PD-L1 expression in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Yao et. al.PubMed 32672418
- Molecular therapy. Nucleic acids 19:814-8262020The MSC-Derived Exosomal lncRNA H19 Promotes Wound Healing in Diabetic Foot Ulcers by Upregulating PTEN via MicroRNA-152-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Li,Song Luan,Jing Chen,Yue Zhou,Tingting Wang,Zhijuan Li,Yili Fu,Aixia Zhai,Changlong BiPubMed 31958697
- Clinical science (London, England : 1979) 133:2265-22822019Tumor-associated macrophage-derived exosomal microRNA-155-5p stimulates intracranial aneurysm formation and macrophage infiltration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengzhe Feng,Xiaoxi Zhang,Li Li,Chuanchuan Wang,Mingtao Feng,Kaijun Zhao,Rui Zhao,Jianmin Liu,Yibin FangPubMed 31657855
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Cy5®
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD63 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08962
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µL for 106 Cells | Notes (or 100 μl of whole blood). |
Associated Products
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4 products for Alternative Version
7 products for Alternative Product
Target data
Function
Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
Alternative names
CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30
Recommended products
Mouse Monoclonal CD63 antibody - conjugated to Cy5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing CD63 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Cy5®
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD63 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08962
- Clone number
- MEM-259
- Purification technique
- Size-exclusion chromatography
- Concentration
- Purification notes
Purified antibody is conjugated with tandem dye PE/Cy5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.
- Biological function summary
CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.
- Pathways
CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.
- Associated diseases and disorders
CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - PE/Cy5® Anti-CD63 antibody [MEM-259] (ab234251), expandable thumbnail](https://content.abcam.com/products/images/pe-cy5-cd63-antibody-mem-259-ab234251--flow-cytometry-img98412.jpg "Flow Cytometry - PE/Cy5® Anti-CD63 antibody [MEM-259] (ab234251)")
Flow Cytometry - PE/Cy5® Anti-CD63 antibody [MEM-259] (ab234251)
Flow cytometric analysis of IgE-activated human peripheral blood labeling CD63 with ab234251 at 4 μl/106 cells.
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