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AB192338

PE/Cy5® Anti-CD64 antibody [10.1]

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(1 Publication)

Mouse Monoclonal CD64 antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing FCGR1A protein.

View Alternative Names

CD64, FCG1, FCGR1, IGFR1, FCGR1A, High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, Fc-gamma RIA, FcRI, FcgammaRIa

2 Images
Flow Cytometry - PE/Cy5® Anti-CD64 antibody [10.1] (AB192338)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD64 antibody [10.1] (AB192338)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab192338 (concentration in sample 0.56 μg/ml).

Flow Cytometry - PE/Cy5® Anti-CD64 antibody [10.1] (AB192338)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD64 antibody [10.1] (AB192338)

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab192338 (concentration in sample 0.56 μg/ml).

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10.1

Isotype

IgG1

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing FCGR1A protein. The exact immunogen used to generate this antibody is proprietary information.

P12314

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.<br><br><a href='/en-us/products/primary-antibodies/pe-cy5-mouse-igg1-kappa-monoclonal-15h6-isotype-control-ab67435'>ab67435</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD64 also known as Fc gamma receptor 1a (FCGR1A) is a high-affinity receptor for the Fc region of immunoglobulin G (IgG). This receptor weighs approximately 72 kDa and is expressed on the surface of immune cells such as macrophages monocytes and activated neutrophils. CD64 protein plays an important role in immune responses by binding to antibodies and mediating processes such as phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). Researchers often use anti-CD64 antibodies and CD64 markers to study its expression in various cellular contexts especially in flow cytometry.
Biological function summary

CD64 expression involves the binding of IgG which serves as a pivotal mechanism to facilitate phagocytosis and clearance of opsonized pathogens. CD64 does not operate solely; it is part of a larger receptor family that includes other Fc gamma receptors like CD16 and CD32. The regulation of CD64 differs from its relatives due to its higher affinity for IgG. This attribute enables CD64 to play a more significant role in triggering immune responses especially during infections and inflammatory processes.

Pathways

CD64 participates in the immune response pathway and is critical in the regulation of inflammatory responses. Key proteins interacting with CD64 in these pathways include the aforementioned CD16 and CD32 forming a network that ensures efficient immune system activation. The interaction with IgG and subsequent signal transduction underpin the receptor's functionality in these pathways harmonizing innate and adaptive immune responses.

CD64 expression correlates strongly with autoimmune disorders and inflammatory conditions. The receptor's role in orchestrating immune cell activation makes it relevant in diseases such as rheumatoid arthritis and sepsis. In rheumatoid arthritis CD64 interacts with immune complexes intensifying inflammation through enhancement of phagocytic activity. In sepsis increased CD64 marker expression on neutrophils can serve as a diagnostic indicator. Related proteins like CD16 and CD32 also participate in these disorders by modulating immune complex handling and cellular activation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.
See full target information FCGR1A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell chemical biology 25:749-760.e9 PubMed29805036

2018

PD Pathway Regulates Human Monocyte Differentiation and Macrophage Function.

Applications

Flow Cyt

Species

Unspecified reactive species

Kimberly Pistorius,Patricia R Souza,Roberta De Matteis,Shani Austin-Williams,Karoline G Primdahl,Anders Vik,Francesca Mazzacuva,Romain A Colas,Raquel M Marques,Trond V Hansen,Jesmond Dalli
View all publications
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Product promise

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