AB95548

PE/Cy5® Anti-CD80 antibody [16-10A1]

Name: PE/Cy5® Anti-CD80 antibody [16-10A1] (ab95548) | Abcam
Brand: Abcam Limited
SKU: AB95548
Price: 330 USD
Availability: InStock

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(2 Publications )

Anti-CD80 antibody [16-10A1] conjugated to PE/Cy5® - suitable for flow cytometry, and reacts with mouse samples. Clone [16-10A1] is a Armenian Hamster monoclonal antibody and one of the most commonly used clones for flow cytometry.

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1 Images

Flow Cytometry - PE/Cy5® Anti-CD80 antibody [16-10A1] (AB95548)

Flow Cyt

Unknown

Flow Cytometry - PE/Cy5® Anti-CD80 antibody [16-10A1] (AB95548)

Staining of 3 day LPS activated C57Bl/6 splenocytes with 0.03 µg of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 µg of ab95548 (filled histogram). Total cells were used for analysis.

387 mFluor™ UV375

mFluor™ UV375 Anti-CD80 antibody [16-10A1]
454 mFluor™ Violet 450

mFluor™ Violet 450 Anti-CD80 antibody [16-10A1]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

16-10A1

Isotype

IgG

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd80 protein. The exact immunogen used to generate this antibody is proprietary information.

Q00609

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.06 µg for 10^5 Cells", "FlowCyt-species-notes": "Cell number should be determined empirically but can range from 105 to 108 cells/test (based on 100 μl test volume).<a href='/en-us/products/primary-antibodies/pe-cy5-armenian-hamster-igg-isotype-control-ab157805'>ab157805</a> - Armenian Hamster polyclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.

Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed : 22450810).

See full target information Cd80

Alternative Names

CD80, B7, Cd80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen

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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Frontiers in microbiology 13:944006 PubMed35992649

2022

Locally generated C3 regulates the clearance of by IFN-γ-primed macrophage through regulation of xenophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Liu,Yan Yan,Xiaoreng Wang,Nannan Chen,Jue Wu

American journal of translational research 13:13135-13146 PubMed34956534

2021

CCR10/CCL27 crosstalk regulates cell metastasis via PI3K-Akt signaling axis in non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yonggang Liu,Ailian Xiao,Biyuan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PE/Cy5® Anti-CD80 antibody [16-10A1]

Flow Cytometry - PE/Cy5® Anti-CD80 antibody [16-10A1] (AB95548)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (2)

Product promise