AB25272

PE/Cy5® Anti-CD90 / Thy1 antibody [G7]

Name: PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (ab25272) | Abcam
Brand: Abcam Limited
SKU: AB25272
Price: 300 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(9 Publications)

Rat Monoclonal CD90 / Thy1 antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 9 publications.

View Alternative Names

CD90, Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, THY1

1 Images

Flow Cytometry - PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (AB25272)

Flow Cyt

Unknown

Flow Cytometry - PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (AB25272)

Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD90 / Thy1 antibody [G7] (PE/Cy5) ab25272 (1 μg/10⁶cells) and Rat Anti-Mouse CD3-epsilon-PE.

660 APC

APC Anti-CD90 / Thy1 antibody [G7]
Biotin

Biotin Anti-CD90 / Thy1 antibody [G7]
519 FITC

FITC Anti-CD90 / Thy1 antibody [G7]
578 PE

PE Anti-CD90 / Thy1 antibody [G7]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

Isotype

IgG2c

Light chain type

kappa

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

ab25272 is specific for Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

ab25272 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. ab25272 has also been reported to promote apoptosis of thymocytes and CTL clones, and to mediate adhesion of thymocytes to thymic stroma.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.1 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose, 0.2% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

See full target information THY1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Experimental physiology 105:876-885 PubMed32052500

2020

microRNA-143-3p regulates odontogenic differentiation of human dental pulp stem cells through regulation of the osteoprotegerin-RANK ligand pathway by targeting RANK.

Applications

Unspecified application

Species

Unspecified reactive species

Changwei Yang,Ru Jia,Qiliang Zuo,Yanfen Zheng,Qianju Wu,Bizhu Luo,Pingting Lin,Lu Yin

IUBMB life 72:665-676 PubMed31889420

2020

Synergistic effects of Rho kinase inhibitor Y-27632 and Noggin overexpression on the proliferation and neuron-like cell differentiation of stem cells derived from human exfoliated deciduous teeth.

Applications

Unspecified application

Species

Unspecified reactive species

Si Yang,Cuijuan Xin,Bo Zhang,Hongbo Zhang,Yunpeng Hao

Molecular medicine reports 19:813-820 PubMed30569163

2018

Effects of menstrual blood‑derived stem cells on endometrial injury repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Hu,Kuangyu Song,Jing Zhang,Yiqiong Zhang,Bu-Zhen Tan

The Journal of experimental medicine 179:785-96 PubMed7906706

1994

Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

A O Hueber,G Raposo,M Pierres,H T He

Journal of immunology (Baltimore, Md. : 1950) 149:1583-92 PubMed1380534

1992

Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death.

Applications

Unspecified application

Species

Unspecified reactive species

D S Ucker,J Meyers,P S Obermiller

The Journal of experimental medicine 173:515-8 PubMed1671083

1991

Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

H T He,P Naquet,D Caillol,M Pierres

Cellular immunology 130:271-80 PubMed1976441

1990

Selection of T cell receptor expression mutants through the functionally linked Ly-6A.

Applications

Unspecified application

Species

Unspecified reactive species

E Sugiyama,A Cantagrel,T Reno,F Stafford-Brady,E T Yeh,J V Bonventre

Journal of immunology (Baltimore, Md. : 1950) 140:1089-94 PubMed2893823

1988

Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3.

Applications

Unspecified application

Species

Unspecified reactive species

L Tentori,D M Pardoll,J C Zuñiga,J Hu-Li,W E Paul,J A Bluestone,A M Kruisbeek

The Journal of experimental medicine 159:716-30 PubMed6142077

1984

T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4.

Applications

Unspecified application

Species

Unspecified reactive species

K C Gunter,T R Malek,E M Shevach

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE/Cy5® Anti-CD90 / Thy1 antibody [G7]

Flow Cytometry - PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (AB25272)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise