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AB95737

PE/Cy5® Anti-TCR gamma + TCR delta antibody [GL3]

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(1 Publication)

Armenian hamster Monoclonal CD3d antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Cd3d protein.

View Alternative Names

CD3d, T3d, Cd3d, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images
Flow Cytometry - PE/Cy5® Anti-TCR gamma + TCR delta antibody [GL3] (AB95737)
  • Flow Cyt

Unknown

Flow Cytometry - PE/Cy5® Anti-TCR gamma + TCR delta antibody [GL3] (AB95737)

ab95737, at 0.5 μg, staining TCR gamma + TCR delta in C57Bl/6 lymph node cells by Flow Cytometry. Cells in the lymphocyte gate were used for analysis.

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

GL3

Isotype

IgG

Conjugation

PE/Cy5®

Excitation/Emission

Ex: 496nm, Em: 667nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Cell preparation containing T-cell surface glycoprotein CD3 gamma chain protein. Database link P11942

Specificity

ab95737 does not react with TCR alpha or beta.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>A final volume of 100 μL is recommended for staining the cell sample.</p><p><a href='/en-us/products/primary-antibodies/pe-cy5-armenian-hamster-igg-isotype-control-ab157805'>ab157805</a> - Armenian Hamster polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCR gamma + TCR delta also known as gamma delta T-cell receptor is a part of the T-cell receptor (TCR) family. It has alternative names like gammadelta TCR or simply TCRδ/γ. The gamma and delta chains form a heterodimer that is part of the cell surface receptor complex. This complex is important for ligand recognition and immune response. The mass of the TCR gamma and TCR delta chains is not specified but they form between ZAP-70 and the CD3 complex on the surface of a subset of T-cells. These receptors are mainly expressed on gamma delta T-cells which are a minor population of T-cells in peripheral blood but more abundant in mucosal tissues and epithelial layers.
Biological function summary

TCR gamma and TCR delta are involved in recognizing antigens without presentation by major histocompatibility complex (MHC) molecules. The receptor complex allows these cells to respond to a diverse range of non-peptide antigens mostly derived from microbial origin including phosphoantigens and lipids. This enables gamma delta T-cells to bridge innate and adaptive immunity. They do not always require antigen processing and can recognize antigens directly contributing efficiently to immune surveillance and early response to infections and cellular stress.

Pathways

TCR gamma and TCR delta are important in the signaling networks that mediate immune responses. They are involved in pathways such as the nuclear factor of activated T-cells (NFAT) and mitogen-activated protein kinase (MAPK) pathways. These pathways promote the activation proliferation and differentiation of T-cells upon antigen recognition. The TCR complex including CD3 plays a role in these signal transduction events and ZAP-70 a protein tyrosine kinase is key for initiating these pathways upon TCR activation.

Mutations or dysregulation in TCR gamma and TCR delta can lead to immune-related conditions. For example abnormalities in these receptors have been linked to autoimmune diseases such as celiac disease and multiple sclerosis. Their role in these disorders is related to faulty recognition and response to autoantigens leading to inappropriate immune responses. Additionally gamma delta T-cells and their receptors are of interest in cancer research as they participate in tumor immunosurveillance. Connections with molecules like CD3 and ZAP-70 facilitate ongoing exploration for therapeutic interventions targeting immune-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly (PubMed : 10935641). Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.
See full target information Cd3d

Additional targets

T-cell surface glycoprotein CD3 gamma chain
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 162:6955-8 PubMed10358132

1999

Cutting edge: anti-CD1 monoclonal antibody treatment reverses the production patterns of TGF-beta 2 and Th1 cytokines and ameliorates listeriosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

G Szalay,C H Ladel,C Blum,L Brossay,M Kronenberg,S H Kaufmann
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