Mouse Monoclonal CD105 antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.
Images
![Flow Cytometry - PE/Cy7® Anti-CD105 antibody [MEM-226] (AB272352), expandable thumbnail](https://content.abcam.com/products/images/pe-cy7-cd105-antibody-mem-226-ab272352--flow-cytometry-img94752.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE/Cy7® Anti-CD105 antibody [MEM-226] (AB272352)")
![Flow Cytometry - PE/Cy7® Anti-CD105 antibody [MEM-226] (AB272352), expandable thumbnail](https://content.abcam.com/products/images/pe-cy7-cd105-antibody-mem-226-ab272352--flow-cytometry-img56974.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE/Cy7® Anti-CD105 antibody [MEM-226] (AB272352)")
Publications
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- Oxidative medicine and cellular longevity 2022:57725092022Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoude Liang,Ruiping Zhou,Xin Liu,Lin You,Chang Chen,Xiaoling Ye,Wei Wei,Jie Liu,Jiawei Dai,Kaixiong Li,Xiangxiang ZhaoPubMed 36105481
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- PE/Cy7®
- Excitation/Emission
- Ex: 565nm, Em: 778nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg for 106 Cells | Notes - |
Target data
Function
Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:21737454, PubMed:23300529). Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed:1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:21737454, PubMed:22347366, PubMed:23300529, PubMed:8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed:21737454, PubMed:22347366, PubMed:23300529).
Alternative names
CD105, END, ENG, Endoglin
Recommended products
Mouse Monoclonal CD105 antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- PE/Cy7®
- Excitation/Emission
- Ex: 565nm, Em: 778nm
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- MEM-226
- Purification technique
- Size-exclusion chromatography
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
- Biological function summary
Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.
- Pathways
CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.
- Associated diseases and disorders
CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - PE/Cy7® Anti-CD105 antibody [MEM-226] (ab272352), expandable thumbnail](https://content.abcam.com/products/images/pe-cy7-cd105-antibody-mem-226-ab272352--flow-cytometry-img94752.jpg "Flow Cytometry - PE/Cy7® Anti-CD105 antibody [MEM-226] (ab272352)")
Flow Cytometry - PE/Cy7® Anti-CD105 antibody [MEM-226] (ab272352)
Separation of HUVEC cells (Red) from HEK-293 (black-dashed) in flow cytometric analysis labeling CD105 using ab272352. (4 μl reagent per milion cells in 100 μl of cell suspension).
![Flow Cytometry - PE/Cy7® Anti-CD105 antibody [MEM-226] (ab272352), expandable thumbnail](https://content.abcam.com/products/images/pe-cy7-cd105-antibody-mem-226-ab272352--flow-cytometry-img56974.jpg "Flow Cytometry - PE/Cy7® Anti-CD105 antibody [MEM-226] (ab272352)")
Flow Cytometry - PE/Cy7® Anti-CD105 antibody [MEM-226] (ab272352)
Flow cytometric analysis of HUVEC labeling CD105 with ab272352 at 4μl reagent per milion cells in 100 μl of cell suspension.
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