Mouse Monoclonal CD20 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing MS4A1 protein.
Images
![Flow Cytometry - PE/Cy7® Anti-CD20 antibody [2H7] (AB234204), expandable thumbnail](https://content.abcam.com/products/images/pe-cy7-cd20-antibody-2h7-ab234204--flow-cytometry-img75380.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE/Cy7® Anti-CD20 antibody [2H7] (AB234204)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Cell preparation containing MS4A1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P11836
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µL for 106 Cells | Notes (or 100 μL whole blood) |
Associated Products
Select an associated product type
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Target data
Function
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602).
Alternative names
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1, CD20
Recommended products
Mouse Monoclonal CD20 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing MS4A1 protein.
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Cell preparation containing MS4A1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P11836
- Clone number
2H7
- Purification technique
Size-exclusion chromatography
- Concentration
- Purification notes
ab234204 is conjugated with tandem dye PE-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
- Biological function summary
CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.
- Pathways
CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.
- Associated diseases and disorders
CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - PE/Cy7® Anti-CD20 antibody [2H7] (ab234204), expandable thumbnail](https://content.abcam.com/products/images/pe-cy7-cd20-antibody-2h7-ab234204--flow-cytometry-img75380.jpg "Flow Cytometry - PE/Cy7® Anti-CD20 antibody [2H7] (ab234204)")
Flow Cytometry - PE/Cy7® Anti-CD20 antibody [2H7] (ab234204)
Surface staining of human peripheral blood for CD20 (lymphocyte gate) using ab234204 at 4 μL/106 cells.
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