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AB234276

PE/Cy7® Anti-CD28 antibody [CD28.2]

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(1 Publication)

Mouse Monoclonal CD28 antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD28 protein.

View Alternative Names

CD28, T-cell-specific surface glycoprotein CD28, TP44

1 Images
Flow Cytometry - PE/Cy7® Anti-CD28 antibody [CD28.2] (AB234276)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-CD28 antibody [CD28.2] (AB234276)

Flow Cytometry analysis of human peripheral blood labeling CD28 with ab234276.

Surface staining, gated for leukocytes.

  • Carrier free

    Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free

  • 660 APC

    APC Anti-CD28 antibody [CD28.2]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD28 antibody [CD28.2]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD28 antibody [CD28.2]

  • Biotin

    Biotin Anti-CD28 antibody [CD28.2]

  • 519 FITC

    FITC Anti-CD28 antibody [CD28.2]

  • 578 PE

    PE Anti-CD28 antibody [CD28.2]

  • 675 PerCP

    PerCP Anti-CD28 antibody [CD28.2]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD28 antibody [CD28.2]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CD28.2

Isotype

IgG1

Conjugation

PE/Cy7®

Excitation/Emission

Ex: 565nm, Em: 778nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD28 protein. The exact immunogen used to generate this antibody is proprietary information.

P10747

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Primary Antibodies

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  • 0
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Primary Antibodies

AB196147

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
ab234276 is conjugated with tandem dye PE-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.
Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 1650475, PubMed : 7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed : 24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed : 12196291, PubMed : 1650475, PubMed : 35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed : 21964608, PubMed : 24665965, PubMed : 7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed : 8617933).. Isoform 3. Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037).
See full target information CD28
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 57:2257-2272.e5 PubMed36182685

2022

Pluripotency factors are repurposed to shape the epigenomic landscape of neural crest cells.

Applications

Unspecified application

Species

Unspecified reactive species

Austin S Hovland,Debadrita Bhattacharya,Ana Paula Azambuja,Dimitrius Pramio,Jacqueline Copeland,Megan Rothstein,Marcos Simoes-Costa
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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