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AB81992

PE/Cy7® Anti-CD3 antibody [UCHT1]

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(3 Publications)

Mouse Monoclonal CD3d antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images
Flow Cytometry - PE/Cy7® Anti-CD3 antibody [UCHT1] (AB81992)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-CD3 antibody [UCHT1] (AB81992)

Human peripheral blood lymphocytes stained with 5μL (0.5μg) ab81992 (solid line) or 0.5μg PE-Cy7-conjugated mouse IgG1 isotype control (dashed line).

  • 660 APC

    APC Anti-CD3 antibody [UCHT1]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD3 antibody [UCHT1]

  • Biotin

    Biotin Anti-CD3 antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 antibody [UCHT1]

  • 519 FITC

    FITC Anti-CD3 epsilon antibody [UCHT1]

  • 578 PE

    PE Anti-CD3 epsilon antibody [UCHT1]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD3 antibody [UCHT1]

  • 675 PerCP

    PerCP Anti-CD3 antibody [UCHT1]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD3 antibody [UCHT1]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD3 antibody [UCHT1]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD3 antibody [UCHT1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UCHT1

Isotype

IgG1

Conjugation

PE/Cy7®

Excitation/Emission

Ex: 565nm, Em: 778nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

Reacts with the 20 kDa e chain of the CD3/TCR complex

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>Use 5μl in a final volume of 100μl.</p><p><a href='/en-us/products/primary-antibodies/pe-cy7-mouse-igg1-kappa-monoclonal-15h6-isotype-control-ab154457'>ab154457</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from TCS
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.88% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Inflammation 48:975-986 PubMed39174864

2024

Optimal Timing of PD-1/PD-L1 Blockade Protects Organ Function During Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xiang,Yuanyang Wang,Langtian Shi,Jinchao Qiu,Lebin Gan,Zhe Xu,Huacai Zhang,Jin Deng,Zhen Wang,Fang Xu,Ling Zeng

Molecular therapy : the journal of the American Society of Gene Therapy 30:1227-1238 PubMed34933101

2021

Tim-3 regulates sepsis-induced immunosuppression by inhibiting the NF-κB signaling pathway in CD4 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Huang,Di Liu,Jianhui Sun,Huacai Zhang,Jing Zhang,Qiang Wang,Lebin Gan,Guoxin Qu,Jinchao Qiu,Jin Deng,Jianxin Jiang,Ling Zeng

European journal of microbiology & immunology 4:123-7 PubMed24883198

2014

Evaluation of immune responses by live infectious bursal disease vaccines to avoid vaccination failures.

Applications

Unspecified application

Species

Unspecified reactive species

P Jakka,Y K Reddy,J J Kirubaharan,N D J Chandran
View all publications
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Product promise

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