Mouse Monoclonal CD33 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD33 protein.
Images
![Flow Cytometry - PE/Cy7® Anti-CD33 antibody [WM53] (AB239310), expandable thumbnail](https://content.abcam.com/products/images/pe-cy7-cd33-antibody-wm53-ab239310--flow-cytometry-img30027.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE/Cy7® Anti-CD33 antibody [WM53] (AB239310)")
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Cell preparation containing CD33 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P20138
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µL for 106 Cells | Notes (or 100 μl of whole blood). |
Associated Products
Select an associated product type
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3 products for Alternative Version
Target data
Function
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).
Alternative names
Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3, SIGLEC3, CD33
Recommended products
Mouse Monoclonal CD33 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD33 protein.
Alternative names
Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3, SIGLEC3, CD33
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Cell preparation containing CD33 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P20138
- Clone number
WM53
- Purification technique
Size-exclusion chromatography
- Concentration
- Purification notes
Purified antibody is conjugated with tandem dye PE/Cy7 ® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - PE/Cy7® Anti-CD33 antibody [WM53] (ab239310), expandable thumbnail](https://content.abcam.com/products/images/pe-cy7-cd33-antibody-wm53-ab239310--flow-cytometry-img30027.jpg "Flow Cytometry - PE/Cy7® Anti-CD33 antibody [WM53] (ab239310)")
Flow Cytometry - PE/Cy7® Anti-CD33 antibody [WM53] (ab239310)
Flow cytometric analysis of human peripheral blood labeling CD33 with ab239310. Surface staining.
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