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AB210372

PE/Cy7® Anti-CD4 antibody [SK3]

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(1 Publication)

Mouse Monoclonal CD4 antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

1 Images
Flow Cytometry - PE/Cy7® Anti-CD4 antibody [SK3] (AB210372)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-CD4 antibody [SK3] (AB210372)

Flow cytometric analysis of Human peripheral blood lymphocytes labeling CD4 with 5 μl (0.06 μg) ab210372 (solid line), compared with 0.06 μg PE/Cy7 Mouse IgG1 isotype control (dashed line).

  • 519 FITC

    FITC Anti-CD4 antibody [SK3]

  • 675 PerCP

    PerCP Anti-CD4 antibody [SK3]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD4 antibody [SK3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SK3

Isotype

IgG1

Light chain type

kappa

Conjugation

PE/Cy7®

Excitation/Emission

Ex: 565nm, Em: 778nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>The antibody has been diluted for use at 5 μl (0.06 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μl.</p>" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

This antibody does not block binding of alternative clone RPA-T4, suggesting that they recognize different epitopes.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
ab210372 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).
See full target information CD4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Blood 129:2526-2536 PubMed28351936

2017

Endoglin: a novel target for therapeutic intervention in acute leukemias revealed in xenograft mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Keina M C Dourado,June Baik,Vanessa K P Oliveira,Miriam Beltrame,Ami Yamamoto,Charles P Theuer,Camila A V Figueiredo,Michael R Verneris,Rita C R Perlingeiro
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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