AB201318

PE/Cy7® Anti-CD64 antibody [10.1]

Name: Anti-CD64 PE/Cy7® antibody [10.1] (ab201318) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB201318
Price: 410 USD
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

Mouse Monoclonal CD64 antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Human, Primates samples. Immunogen corresponding to Cell preparation containing FCGR1A protein.

View Alternative Names

CD64, FCG1, FCGR1, IGFR1, FCGR1A, High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, Fc-gamma RIA, FcRI, FcgammaRIa

2 Images

Flow Cytometry - PE/Cy7® Anti-CD64 antibody [10.1] (AB201318)

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-CD64 antibody [10.1] (AB201318)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab201318 (4 μl reagent / 100 μl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-CD64 antibody [10.1] (AB201318)

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab201318 (4 μl reagent / 100 μl of peripheral whole blood).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10.1

Isotype

IgG1

Conjugation

PE/Cy7®

Excitation/Emission

Ex: 565nm, Em: 778nm

Carrier free

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing FCGR1A protein. The exact immunogen used to generate this antibody is proprietary information.

P12314

Specificity

ab201318 recognizes the alpha subunit of CD64.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.</p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension.</p>" } } }

style

left-column

You may be interested in:

AB300330

Human CD64 ELISA Kit

0 Reviews

View product

Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: 99% PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD64 also known as Fc gamma receptor 1a (FCGR1A) is a high-affinity receptor for the Fc region of immunoglobulin G (IgG). This receptor weighs approximately 72 kDa and is expressed on the surface of immune cells such as macrophages monocytes and activated neutrophils. CD64 protein plays an important role in immune responses by binding to antibodies and mediating processes such as phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). Researchers often use anti-CD64 antibodies and CD64 markers to study its expression in various cellular contexts especially in flow cytometry.

Biological function summary

CD64 expression involves the binding of IgG which serves as a pivotal mechanism to facilitate phagocytosis and clearance of opsonized pathogens. CD64 does not operate solely; it is part of a larger receptor family that includes other Fc gamma receptors like CD16 and CD32. The regulation of CD64 differs from its relatives due to its higher affinity for IgG. This attribute enables CD64 to play a more significant role in triggering immune responses especially during infections and inflammatory processes.

Pathways

CD64 participates in the immune response pathway and is critical in the regulation of inflammatory responses. Key proteins interacting with CD64 in these pathways include the aforementioned CD16 and CD32 forming a network that ensures efficient immune system activation. The interaction with IgG and subsequent signal transduction underpin the receptor's functionality in these pathways harmonizing innate and adaptive immune responses.

CD64 expression correlates strongly with autoimmune disorders and inflammatory conditions. The receptor's role in orchestrating immune cell activation makes it relevant in diseases such as rheumatoid arthritis and sepsis. In rheumatoid arthritis CD64 interacts with immune complexes intensifying inflammation through enhancement of phagocytic activity. In sepsis increased CD64 marker expression on neutrophils can serve as a diagnostic indicator. Related proteins like CD16 and CD32 also participate in these disorders by modulating immune complex handling and cellular activation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.

See full target information FCGR1A

style

left-column

style

left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

style

left-column

style

primary-antibodies

cd64-antibody-epr4623-low-endotoxin-azide-free-ab246692

(0 reviews)

style

bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com