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Armenian hamster Monoclonal CD69 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Cell preparation containing Cd69 protein.

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Images

Flow Cytometry - PE/Cy7® Anti-CD69 antibody [H1.2F3] (AB25529), expandable thumbnail

Key facts

Isotype

IgG

Host species

Armenian hamster

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Storage buffer

pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing Cd69 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P37217

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

0.2 µg for 106 Cells

Notes

-

Associated Products

Select an associated product type

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2 products for Alternative Version

Target data

Function

Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types (PubMed:20696842, PubMed:38540778). Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells (By similarity). Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage (PubMed:20696842). Acts also via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells. Acts as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T-lymphocytes and negatively regulates the inflammatory response by inducing the expression of PDCD1 through the activation of NFAT. Participates in adipose tissue-derived mesenchymal stem cells (ASCs)-mediated protection against P. aeruginosa infection. Mechanistically, specifically recognizes P. aeruginosa to promote ERK1 activation, followed by granulocyte-macrophage colony-stimulating factor (GM-CSF) and other inflammatory cytokines secretion (By similarity). In eosinophils, induces IL-10 production through the ERK1/2 pathway (PubMed:38540778). Negatively regulates the chemotactic responses of effector lymphocytes and dendritic cells (DCs) to sphingosine 1 phosphate/S1P by acting as a S1PR1 receptor agonist and facilitating the internalization and degradation of the receptor (PubMed:16525420).

Alternative names

Recommended products

Armenian hamster Monoclonal CD69 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Cell preparation containing Cd69 protein.

Key facts

Isotype

IgG

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing Cd69 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P37217
Clone number

H1.2F3

Purification technique

Affinity purification

Specificity

ab25529 recognises mouse CD69/Very Early Activation Antigen (VEA).

Light chain type

lambda

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

Notes

This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD69 also known as Activation-Induced C-type Lectin or Early T-cell Activation Antigen is a type II transmembrane protein with a mass of approximately 28-32 kDa. It is expressed mainly on activated leukocytes including T cells B cells and natural killer (NK) cells. Expression occurs rapidly after cell activation making CD69 a robust early marker used in flow cytometry analysis like with CD69 FITC or CD69 PE conjugates to study immune cell activation. The mouse version of CD69 serves as an important model for understanding its function in human biology.

Biological function summary

CD69 functions as a signaling molecule during the immune response influencing both cell proliferation and cytokine production. It is a member of the C-type lectin superfamily and operates as a homodimer on the cell surface. CD69 helps modulate the migration of T cells to inflammatory sites by affecting sphingosine-1-phosphate receptor 1 (S1P1) downregulation directing their tissue retention and primary response roles.

Pathways

The protein plays an important role in the regulation of the NF-kB and JAK-STAT signaling pathways. These are important for immune cell communication and response and CD69 interacts with proteins like the IL-2 receptor complex in these pathways. Additionally its cross-linking can lead to altered expression of cytokines and chemokines impacting overall immune function.

Associated diseases and disorders

Altered CD69 expression links to conditions like autoimmune diseases and chronic inflammation. Its involvement in rheumatoid arthritis shows how CD69 may regulate abnormal immune responses. Furthermore the modulation of CD69 expression can be associated with cancer progression highlighting connections to proteins like FN50 and FN61 which participate in tumor immunity and metastasis control.

Product promise

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1 product image

  • Flow Cytometry - PE/Cy7® Anti-CD69 antibody [H1.2F3] (ab25529), expandable thumbnail

    Flow Cytometry - PE/Cy7® Anti-CD69 antibody [H1.2F3] (ab25529)

    Flow cytometry analysis of Con-A stimulated BALB/c mouse splenocutes with ab25529.

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Product protocols

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