Armenian hamster Monoclonal CD69 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Cell preparation containing Cd69 protein.
IgG
Armenian hamster
PE
Ex: 480;565nm, Em: 578nm
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Liquid
Monoclonal
Flow Cyt | |
---|---|
Mouse | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.2 µg for 106 Cells | Notes - |
Select an associated product type
Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types (PubMed:20696842, PubMed:38540778). Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells (By similarity). Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage (PubMed:20696842). Acts also via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells. Acts as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T-lymphocytes and negatively regulates the inflammatory response by inducing the expression of PDCD1 through the activation of NFAT. Participates in adipose tissue-derived mesenchymal stem cells (ASCs)-mediated protection against P. aeruginosa infection. Mechanistically, specifically recognizes P. aeruginosa to promote ERK1 activation, followed by granulocyte-macrophage colony-stimulating factor (GM-CSF) and other inflammatory cytokines secretion (By similarity). In eosinophils, induces IL-10 production through the ERK1/2 pathway (PubMed:38540778). Negatively regulates the chemotactic responses of effector lymphocytes and dendritic cells (DCs) to sphingosine 1 phosphate/S1P by acting as a S1PR1 receptor agonist and facilitating the internalization and degradation of the receptor (PubMed:16525420).
Early activation antigen CD69, Cd69
Armenian hamster Monoclonal CD69 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Cell preparation containing Cd69 protein.
IgG
Armenian hamster
PE
Ex: 480;565nm, Em: 578nm
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Liquid
Monoclonal
H1.2F3
Affinity purification
ab25529 recognises mouse CD69/Very Early Activation Antigen (VEA).
lambda
Blue Ice
+4°C
+4°C
Store in the dark
This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells.
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This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
This supplementary information is collated from multiple sources and compiled automatically.
CD69 also known as Activation-Induced C-type Lectin or Early T-cell Activation Antigen is a type II transmembrane protein with a mass of approximately 28-32 kDa. It is expressed mainly on activated leukocytes including T cells B cells and natural killer (NK) cells. Expression occurs rapidly after cell activation making CD69 a robust early marker used in flow cytometry analysis like with CD69 FITC or CD69 PE conjugates to study immune cell activation. The mouse version of CD69 serves as an important model for understanding its function in human biology.
CD69 functions as a signaling molecule during the immune response influencing both cell proliferation and cytokine production. It is a member of the C-type lectin superfamily and operates as a homodimer on the cell surface. CD69 helps modulate the migration of T cells to inflammatory sites by affecting sphingosine-1-phosphate receptor 1 (S1P1) downregulation directing their tissue retention and primary response roles.
The protein plays an important role in the regulation of the NF-kB and JAK-STAT signaling pathways. These are important for immune cell communication and response and CD69 interacts with proteins like the IL-2 receptor complex in these pathways. Additionally its cross-linking can lead to altered expression of cytokines and chemokines impacting overall immune function.
Altered CD69 expression links to conditions like autoimmune diseases and chronic inflammation. Its involvement in rheumatoid arthritis shows how CD69 may regulate abnormal immune responses. Furthermore the modulation of CD69 expression can be associated with cancer progression highlighting connections to proteins like FN50 and FN61 which participate in tumor immunity and metastasis control.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Flow cytometry analysis of Con-A stimulated BALB/c mouse splenocutes with ab25529.
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