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AB234229

PE/Cy7® Anti-CD80 antibody [MEM-233]

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(2 Publications)

Mouse Monoclonal CD80 antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD80.

View Alternative Names

CD80, CD28LG, CD28LG1, LAB7, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7

1 Images
Flow Cytometry - PE/Cy7® Anti-CD80 antibody [MEM-233] (AB234229)
  • Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-CD80 antibody [MEM-233] (AB234229)

Surface staining of CD80-transfected P815 cells with ab234229. Gated on singlets.

  • Unconjugated

    Anti-CD80 antibody [MEM-233]

  • 660 APC

    APC Anti-CD80 antibody [MEM-233]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD80 antibody [MEM-233]

  • 519 FITC

    FITC Anti-CD80 antibody [MEM-233]

  • 578 PE

    PE Anti-CD80 antibody [MEM-233]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD80 antibody [MEM-233]

  • 675 PerCP

    PerCP Anti-CD80 antibody [MEM-233]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-233

Isotype

IgG1

Conjugation

PE/Cy7®

Excitation/Emission

Ex: 565nm, Em: 778nm

Carrier free

No

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CD80. The exact immunogen used to generate this antibody is proprietary information.

P33681

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 38467718). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed : 10438913). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 10583602, PubMed : 11279502). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed : 12121659). Also acts as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed : 36727298). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity).. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD80
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:23 PubMed39775116

2025

Exosomal NEDD4L derived from HG+oxLDL-induced vascular endothelial cells accelerates macrophage M1 polarization and oxLDL uptake by ubiquitinating IκBα and PPARγ.

Applications

Unspecified application

Species

Unspecified reactive species

Guozhu Chen,Yisong Pei,Peng Jiang,Qiaoling Ye,Zulong Xie,Laxman Gyawali

Virulence 15:2368080 PubMed38899573

2024

Screening optimal DC-targeting peptide to enhance the immune efficacy of recombinant expressing RHDV VP60.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Xia,Xiao Lu,Deming Kong,Tiantian Guo,Yueyi Gao,Lingxiang Xin,Yanping Jiang,Xiaona Wang,Zhifu Shan,Jiaxuan Li,Han Zhou,Wen Cui,Xinyuan Qiao,Lijie Tang,Yijing Li,Li Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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