AB218761

PE/Cy7® Anti-F4/80 antibody [BM8.1]

Name: Anti-F4/80 PE/Cy7® antibody [BM8.1] (ab218761) Rat monoclonal IgG2b | Abcam
Brand: Abcam Limited
SKU: AB218761
Price: 525 USD
Availability: InStock

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(4 Publications)

Rat Monoclonal F4/80 antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

EMR1, TM7LN3, ADGRE1, Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

1 Images

Flow Cytometry - PE/Cy7® Anti-F4/80 antibody [BM8.1] (AB218761)

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-F4/80 antibody [BM8.1] (AB218761)

Flow Cytometric analysis of C57Bl/6 bone marrow cells labeling F4/80 with APC Anti-Mouse CD11b and 0.25 μg ab218761 (right panel) or 0.25 μg PE-Cyanine7 Rat IgG2b isotype control (left panel).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

BM8.1

Isotype

IgG2b

Light chain type

kappa

Conjugation

PE/Cy7®

Excitation/Emission

Ex: 565nm, Em: 778nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab218761 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information ADGRE1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 29:61 PubMed38671352

2024

Mitochondrial (mt)DNA-cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling promotes pyroptosis of macrophages via interferon regulatory factor (IRF)7/IRF3 activation to aggravate lung injury during severe acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqiu Peng,Yuxi Yang,Yingying Li,Tingjuan Shi,Ning Xu,Ruixia Liu,Yingyi Luan,Yongming Yao,Chenghong Yin

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2105650 PubMed35603963

2022

Hybrid Biomaterial Initiates Refractory Wound Healing via Inducing Transiently Heightened Inflammatory Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Liu,Geng Dou,Zihan Li,Xiangdong Wang,Ronghua Jin,Yao Liu,Huijuan Kuang,Xiaoyao Huang,Xiaoxue Yang,Xiaoshan Yang,Siying Liu,Meiling Wu,Hao Guo,Feng Ding,Haokun Xu,Shiyu Liu,Yan Jin,Kun Xuan

Science advances 6:eaba2987 PubMed32832662

2020

Chimeric apoptotic bodies functionalized with natural membrane and modular delivery system for inflammation modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Dou,Ran Tian,Xuemei Liu,Pingyun Yuan,Qianwen Ye,Jin Liu,Siying Liu,Jun Zhou,Zhihong Deng,Xin Chen,Shiyu Liu,Yan Jin

Cancer science 109:279-288 PubMed29224228

2017

Newcastle disease virus co-expressing interleukin 7 and interleukin 15 modified tumor cells as a vaccine for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Xu,Qing Sun,Yu Mei,Yonghao Liu,Lixiang Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE/Cy7® Anti-F4/80 antibody [BM8.1]

Flow Cytometry - PE/Cy7® Anti-F4/80 antibody [BM8.1] (AB218761)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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