AB210232

PE/Cy7® Anti-FOXP3 antibody [3G3]

Name: Anti-FOXP3 PE/Cy7® antibody [3G3] (ab210232) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB210232

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(7 Publications)

Mouse Monoclonal FOXP3 antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 7 publications.

View Alternative Names

IPEX, JM2, FOXP3, Forkhead box protein P3, Scurfin

1 Images

Flow Cytometry (Intracellular) - PE/Cy7® Anti-FOXP3 antibody [3G3] (AB210232)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE/Cy7® Anti-FOXP3 antibody [3G3] (AB210232)

C57Bl/6 splenocytes were stained with an APC anti-mouse CD4, followed by intracellular staining with 0.125 ugab20232 (right panel) or 0.125 ug PE-Cy7 Mouse IgG1 isotype control (left panel).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3G3

Isotype

IgG1

Light chain type

kappa

Conjugation

PE/Cy7®

Excitation/Emission

Ex: 565nm, Em: 778nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

This antibody was purified from tissue culture supernatant via affinity chromatography. It was conjugated under optimal conditions, with unreacted dye removed from the preparation

Storage buffer

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.

Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the FOXP3 gene is a transcriptional regulator critical for the development and inhibitory function of regulatory T-cells (Treg). It plays an essential role in immune system homeostasis by ensuring the suppressive function and stability of Treg cells and directly influencing conventional T-cells' expansion and function. FOXP3 can function as either a transcriptional repressor or activator, depending on its interactions with other factors, such as transcription factors, histone acetylases, and deacetylases. It co-activates genes like CTLA4 and TNFRSF18 and represses cytokine genes, including interleukin-2 (IL2) and interferon-gamma (IFNG), and inhibits cytokine production by repressing the activity of transcription factors RELA and NFATC2. FOXP3's association with histone acetylase KAT5 and deacetylase HDAC7 mediates IL2 repression, while its interaction with transcription factor RUNX1 modulates the expression of TNFRSF18, IL2RA, CTLA4, IL2, and IFNG. It also antagonizes RORC to inhibit the differentiation of IL17 producing helper T-cells (Th17), leading to reduced IL17 expression and promoting Treg development, while inhibiting the transcriptional activator activity of RORA. This supplementary information is collated from multiple sources and compiled automatically.

See full target information FOXP3

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 6:104021 PubMed40811056

2025

Protocol to develop a 3D mouse autologous lung tumor-immune spheroid co-culture model to advance radiotherapy-based treatments.

Applications

Unspecified application

Species

Unspecified reactive species

Sergio León,Sofía Torres Quintas,Nerea Otegui,Miriam Redrado,José Javier Aristu,María José Oliveira,Alfonso Calvo,Flávia Castro,Diego Serrano

Acta biochimica et biophysica Sinica : PubMed40551711

2025

Single-cell and bulk transcriptome analysis unveils a ligand-receptor-based signature for prognostication and reveals that TREM1 controls the malignant behaviors of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiemin Zhang,Qian Huang,Yingying Zheng,Jianqing Tang,Naling Kang,Yurui Liu,Dawu Zeng

Journal of nanobiotechnology 22:638 PubMed39420389

2024

Novel combination therapy using recombinant oncolytic adenovirus silk hydrogel and PD-L1 inhibitor for bladder cancer treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqiang Zhang,Jianqiang Zhang,Jingwei Zhang,Jing Chu,Zhenxing Zhang

Journal for immunotherapy of cancer 9: PubMed34281988

2021

CD137 and PD-L1 targeting with immunovirotherapy induces a potent and durable antitumor immune response in glioblastoma models.

Applications

Unspecified application

Species

Unspecified reactive species

Montserrat Puigdelloses,Marc Garcia-Moure,Sara Labiano,Virginia Laspidea,Marisol Gonzalez-Huarriz,Marta Zalacain,Lucia Marrodan,Naiara Martinez-Velez,Daniel De la Nava,Iker Ausejo,Sandra Hervás-Stubbs,Guillermo Herrador,ZhiHong Chen,Dolores Hambardzumyan,Ana Patino Garcia,Hong Jiang,Candelaria Gomez-Manzano,Juan Fueyo,Jaime Gállego Pérez-Larraya,Marta Alonso

International journal of molecular medicine 48: PubMed34036377

2021

Propofol maintains Th17/Treg cell balance and reduces inflammation in rats with traumatic brain injury via the miR‑145‑3p/NFATc2/NF‑κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Can Cui,Dengwen Zhang,Ke Sun,Haifeng Li,Liqian Xu,Gen Lin,Yuanbo Guo,Jiaqi Hu,Jieyuan Chen,Lidan Nong,Yujin Cai,Dongnan Yu,Wei Yang,Peng Wang,Yi Sun

Frontiers in immunology 11:1934 PubMed33013847

2020

Mechanisms of M2 Macrophage-Derived Exosomal Long Non-coding RNA PVT1 in Regulating Th17 Cell Response in Experimental Autoimmune Encephalomyelitisa.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wu,Jinjin Xia,Donghui Li,Ying Kang,Wei Fang,Peng Huang

The Journal of allergy and clinical immunology 144:945-961.e9 PubMed31356919

2019

Tolerogenic signaling of alveolar macrophages induces lung adaptation to oxidative injury.

Applications

Unspecified application

Species

Unspecified reactive species

Benoit Allard,Alice Panariti,Erwan Pernet,Jeffrey Downey,Satoshi Ano,Marieme Dembele,Emily Nakada,Utako Fujii,Toby K McGovern,William S Powell,Maziar Divangahi,James G Martin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE/Cy7® Anti-FOXP3 antibody [3G3]

Flow Cytometry (Intracellular) - PE/Cy7® Anti-FOXP3 antibody [3G3] (AB210232)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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