AB25514

PE/Cy7® Anti-Ly6g antibody [RB6-8C5]

Name: PE/Cy7® Anti-Ly6g antibody [RB6-8C5] (ab25514) | Abcam
Brand: Abcam Limited
SKU: AB25514
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(13 Publications)

Rat Monoclonal Ly6g antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 13 publications.

View Alternative Names

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g, Lymphocyte antigen 6C1, Ly-6C1, Ly6c1

1 Images

Flow Cytometry - PE/Cy7® Anti-Ly6g antibody [RB6-8C5] (AB25514)

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-Ly6g antibody [RB6-8C5] (AB25514)

C57Bl/6 bone marrow cells stained with 0.25μg ab25514 (solid line) or 0.25μg PE-Cy7-conjugated rat IgG2b isotype control (dashed line).

Unconjugated

Anti-Ly6g + Ly6c antibody [RB6-8C5]
redFluor™ 710

redFluor™ 710 Anti-Ly6g antibody [RB6-8C5]
578 PE

PE Anti-Ly6g antibody [RB6-8C5]
660 APC

APC Anti-Ly6g antibody [RB6-8C5]
519 FITC

FITC Anti-Ly6g antibody [RB6-8C5]
450 violetFluor™ 450

violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RB6-8C5

Isotype

IgG2b

Light chain type

kappa

Conjugation

PE/Cy7®

Excitation/Emission

Ex: 565nm, Em: 778nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/pe-cy7-rat-igg2b-kappa-monoclonal-klh-g2b-1-2-isotype-control-ab154461'>ab154461</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

style

left-column

Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from TCS

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.88% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.

Biological function summary

Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

Pathways

Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Ly6g

Additional targets

Ly6c1

style

left-column

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 36:84-99.e8 PubMed31287994

2019

Mubritinib Targets the Electron Transport Chain Complex I and Reveals the Landscape of OXPHOS Dependency in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Irène Baccelli,Yves Gareau,Bernhard Lehnertz,Stéphane Gingras,Jean-François Spinella,Sophie Corneau,Nadine Mayotte,Simon Girard,Mélanie Frechette,Valérie Blouin-Chagnon,Koryne Leveillé,Isabel Boivin,Tara MacRae,Jana Krosl,Clarisse Thiollier,Vincent-Philippe Lavallée,Evgeny Kanshin,Thierry Bertomeu,Jasmin Coulombe-Huntington,Corinne St-Denis,Marie-Eve Bordeleau,Geneviève Boucher,Philippe P Roux,Sébastien Lemieux,Mike Tyers,Pierre Thibault,Josée Hébert,Anne Marinier,Guy Sauvageau

International journal of molecular sciences 20: PubMed31252620

2019

The Psoriasis Therapeutic Potential of a Novel Short Laminin Peptide C16.

Applications

Unspecified application

Species

Unspecified reactive species

Tsung-Chuan Ho,Shu-I Yeh,Show-Li Chen,Yeou-Ping Tsao

Journal of immunological methods 197:139-50 PubMed8890901

1996

Flow cytometric identification of murine neutrophils and monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

E Lagasse,I L Weissman

Journal of virology 70:898-904 PubMed8551629

1996

Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea.

Applications

Unspecified application

Species

Unspecified reactive species

T M Tumpey,S H Chen,J E Oakes,R N Lausch

Journal of leukocyte biology 57:827-31 PubMed7790763

1995

Neutrophils are essential for resolution of primary and secondary infection with Listeria monocytogenes.

Applications

Unspecified application

Species

Unspecified reactive species

A L Rakhmilevich

Journal of immunology (Baltimore, Md. : 1950) 152:1836-46 PubMed8120393

1994

Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection.

Applications

Unspecified application

Species

Unspecified reactive species

C J Czuprynski,J F Brown,N Maroushek,R D Wagner,H Steinberg

The Journal of experimental medicine 179:259-68 PubMed8270870

1994

Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

J W Conlan,R J North

Journal of immunology (Baltimore, Md. : 1950) 151:2399-408 PubMed8360469

1993

Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.

Applications

Unspecified application

Species

Unspecified reactive species

T J Fleming,M L Fleming,T R Malek

The Journal of experimental medicine 178:151-61 PubMed7686211

1993

Regression of an established tumor genetically modified to release granulocyte colony-stimulating factor requires granulocyte-T cell cooperation and T cell-produced interferon gamma.

Applications

Unspecified application

Species

Unspecified reactive species

A Stoppacciaro,C Melani,M Parenza,A Mastracchio,C Bassi,C Baroni,G Parmiani,M P Colombo

Journal of immunology (Baltimore, Md. : 1950) 147:22-8 PubMed1711076

1991

Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells.

Applications

Unspecified application

Species

Unspecified reactive species

K Hestdal,F W Ruscetti,J N Ihle,S E Jacobsen,C M Dubois,W C Kopp,D L Longo,J R Keller

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE/Cy7® Anti-Ly6g antibody [RB6-8C5]

Flow Cytometry - PE/Cy7® Anti-Ly6g antibody [RB6-8C5] (AB25514)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (13)

Product promise