Mouse Monoclonal NCAM1 antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - PE/Cy7® Anti-NCAM1 antibody [MY31] (AB210305), expandable thumbnail](https://content.abcam.com/products/images/pe-cy7-ncam1-antibody-my31-ab210305--flow-cytometry-img13172.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - PE/Cy7® Anti-NCAM1 antibody [MY31] (AB210305)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE/Cy7®
- Excitation/Emission
- Ex: 565nm, Em: 778nm
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
| Chimpanzee | Predicted |
| Cynomolgus monkey | Predicted |
| Rhesus monkey | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µL for 105-108 Cells | Notes The antibody has been diluted for use at 5 μl (0.5 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μl. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Cynomolgus monkey, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.
Alternative names
CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1
Recommended products
Mouse Monoclonal NCAM1 antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- PE/Cy7®
- Excitation/Emission
- Ex: 565nm, Em: 778nm
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- MY31
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
- Purification notes
ab210305 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
- Biological function summary
NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.
- Pathways
The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.
- Associated diseases and disorders
NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Flow Cytometry - PE/Cy7® Anti-NCAM1 antibody [MY31] (ab210305), expandable thumbnail](https://content.abcam.com/products/images/pe-cy7-ncam1-antibody-my31-ab210305--flow-cytometry-img13172.jpg "Flow Cytometry - PE/Cy7® Anti-NCAM1 antibody [MY31] (ab210305)")
Flow Cytometry - PE/Cy7® Anti-NCAM1 antibody [MY31] (ab210305)
Flow cytometric analysis of Human peripheral blood lymphocytes stained with an APC Anti-Human CD3, followed by labeling of NCAM with 5 μl (0.5 μg) ab210305 (right panel), compared with 0.5 μg PE/Cy7 Mouse IgG1 isotype control (left panel).
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