AB234270

PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5]

Name: PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5] (ab234270) | Abcam
Brand: Abcam Limited
SKU: AB234270
Price: 410 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal p75 NGF Receptor antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human NGFR.

View Alternative Names

CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor

1 Images

Flow Cytometry - PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5] (AB234270)

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5] (AB234270)

Flow cytometric analysis of REH cells labeling p75 NGF Receptor with ab234270 at 4 μl/10⁶ cells (red) as compared to a blank control (blue).

Carrier free

Anti-p75 NGF Receptor antibody [NGFR5] - BSA and Azide free
Unconjugated

Anti-p75 NGF Receptor antibody [NGFR5]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NGFR5

Isotype

IgG1

Conjugation

PE/Cy7®

Excitation/Emission

Ex: 565nm, Em: 778nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human NGFR.

P08138

Epitope

The epitope is localized within ammino acids 1 - 160.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "(or 100 μl of whole blood)." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "(or 100 μl of whole blood)." }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "(or 100 μl of whole blood)." }, "Cat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Ferret": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Primates": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purified antibody is conjugated with tandem dye PE/Cy7 ® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage buffer

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The p75 NGF receptor also known as p75 NGFR or anti-NTR is a transmembrane protein with a molecular mass of approximately 75 kDa. It is a member of the tumor necrosis factor receptor superfamily. This receptor is widely expressed in the nervous system particularly in neurons glial cells and Schwann cells. The p75 receptor primarily binds to neurotrophins such as nerve growth factor (NGF) influencing neuronal survival apoptosis and growth.

Biological function summary

The p75 receptor functions as an important part of a signaling complex that mediates diverse cellular responses. It often forms complexes with other receptors like TrkA but can also signal independently. In particular the p75 protein can form a biological chimera with different co-receptors influencing its downstream effects. This makes it significant in processes like apoptosis and neuronal differentiation as well as influencing neurotrophin-mediated signaling.

Pathways

The p75 receptor plays an important role in pathways involving neuronal survival and apoptosis. One key pathway involves the nerve growth factor (NGF) signaling pathway where p75 collaborates with Trk receptors enhancing or inhibiting NGF signaling based on cellular context. Another pathway is the JNK signaling pathway where the p75 receptor interacts with proteins like sortilin contributing to apoptosis and stress responses in neurons. These interactions determine the outcome of neurotrophic signaling highlighting its role in cell fate decisions.

P75 receptor is associated with several neurodegenerative conditions and cancers. Its involvement in Alzheimer's disease is significant because altered p75 expression and function can contribute to neurodegeneration through apoptosis pathways. This receptor is also linked to glioblastoma where it might promote tumor progression in coordination with proteins such as sortilin. These links make p75 receptor a target of interest for therapeutic intervention in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed : 24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed : 26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed : 23785138). Together with BFAR negatively regulates NF-kappa-B and JNK-related signaling pathways (PubMed : 22566094).

See full target information NGFR

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 57:2257-2272.e5 PubMed36182685

2022

Pluripotency factors are repurposed to shape the epigenomic landscape of neural crest cells.

Applications

Unspecified application

Species

Unspecified reactive species

Austin S Hovland,Debadrita Bhattacharya,Ana Paula Azambuja,Dimitrius Pramio,Jacqueline Copeland,Megan Rothstein,Marcos Simoes-Costa

Cells 11: PubMed35406690

2022

Single-Cell Molecular Characterization to Partition the Human Glioblastoma Tumor Microenvironment Genetic Background.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Lessi,Sara Franceschi,Mariangela Morelli,Michele Menicagli,Francesco Pasqualetti,Orazio Santonocito,Carlo Gambacciani,Francesco Pieri,Filippo Aquila,Paolo Aretini,Chiara Maria Mazzanti

Frontiers in cellular neuroscience 13:523 PubMed31849612

2019

BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development.

Applications

Unspecified application

Species

Unspecified reactive species

Sizhou Huang,Yi Wang,Lingfei Luo,Xiaoqing Li,Xianqing Jin,Shuangshuang Li,Xiaoping Yu,Min Yang,Zhenhua Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5]

Flow Cytometry - PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5] (AB234270)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise